Determination of C-reactive protein by PAMAM decorated ITO based disposable biosensing system: A new immunosensor design from an old molecule

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dc.authorid0000-0002-3153-1328
dc.authorscopusid56800873300
dc.authorscopusid6506764918
dc.authorwosidKaraboga, Munteha Nur Sonuc/ABA-6419-2020
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.contributor.authorKaraboğa, Munteha Nur Sonuç
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:48:42Z
dc.date.available2022-05-11T14:48:42Z
dc.date.issued2018
dc.departmentYüksekokullar, Sağlık Yüksekokulu, Beslenme ve Diyetetik Bölümü
dc.description.abstractThis paper demonstrates a new and sensitive electrochemical immunosensor for the analysis of C-reactive protein, an important marker of inflammation. Indium Tin Oxide (ITO) disposable sheets were modified by using 11-cyanoundecyltrimethoxysilane (CUTMS) and PAMAM dendrimers (G:1 amino surfaces) for the first time to immobilize the anti-CRP antibody via covalent interactions. Cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS), as well as square wave voltammetry (SWV) methods, were applied to characterize the immobilization stages of anti-CRP and to determine the CRP concentrations. Charge transfer resistance changes were highly linear and sensitive to CRP concentration in the range 21-6148 fg mL(-1) and were associated with a limit of detection of 0.34 fg mL(-1). The system had acceptable repeatability (6A5%, n = 18) and good storage stability (4.5% loss after 6 weeks). Moreover, the binding characterization of CRP to anti-CRP was monitored by a single frequency impedance technique. The amount of CRP in human serum samples was analyzed with a fabricated biosensor to determine the feasibility of the biosensing system for medical purposes. We suggest that 11-CUTMS, a new silanization agent, is ideal for biosensor applications.
dc.identifier.doi10.1016/j.talanta.2018.04.051
dc.identifier.endpage168
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid29784344
dc.identifier.scopus2-s2.0-85046101104
dc.identifier.scopusqualityQ1
dc.identifier.startpage162
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2018.04.051
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10675
dc.identifier.volume186
dc.identifier.wosWOS:000435048800023
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKaraboğa, Munteha Nur Sonuç
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofTalanta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject11-cyarioundecyltrimethoxysilane
dc.subjectC-reactive protein (CRP)
dc.subjectBiosensor
dc.subjectElectrochemical impedance spectroscopy (EIS)
dc.subjectPAMAM dendrimers
dc.subjectLabel-Free Detection
dc.subjectCognitive Function
dc.subjectCrp Detection
dc.subjectSerum
dc.subjectImmobilization
dc.subjectAssociation
dc.subjectDiagnosis
dc.subjectDisease
dc.subjectLeptin
dc.titleDetermination of C-reactive protein by PAMAM decorated ITO based disposable biosensing system: A new immunosensor design from an old molecule
dc.typeArticle

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