Fabrication of a highly sensitive conductive copolymer layer modified immunosensing tool for cytokeratin-19 fragment detection

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dc.authoridAYDIN, Elif Burcu/0000-0002-1982-4128
dc.contributor.authorAydin, Elif Burcu
dc.contributor.authorAydin, Muhammet
dc.contributor.authorSezginturk, Mustafa Kemal
dc.date.accessioned2024-10-29T17:58:28Z
dc.date.available2024-10-29T17:58:28Z
dc.date.issued2024
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractDesign of a new and highly selective impedimetric biosensor based on the immobilization of a fragment of cytokeratin subunit 19 (CYFRA 21-1)-specific antibodies onto a conductive copolymer layer (poly(epoxy-substituted thiophene)-co-poly(3,4-ethylenedioxythiophene), CCL)-coated indium tin oxide (ITO) for the determination of CYFRA 21-1 in serum samples was reported. CCL was applied as a working electrode-modifying reagent to prepare the surface of the working ITO electrode, and CYFRA 21-1-specific antibodies bound to epoxy ends present in the copolymer layer matrix. The impedimetric response of the immunosensor to CYFRA 21-1 was recorded, and the signaling mechanism of the suggested immunosensor was based on an increase in the charge transfer resistance in the presence of target CYFRA 21-1. The developed biosensor exhibited excellent selectivity for CYFRA 21-1, and it could detect CYFRA 21-1 in a linear dynamic range from 0.0125 to 35 pg/mL with a limit of quantification (LOQ) of 12.25 fg/mL. The designed biosensor showed good storage stability, high sensitivity, suitable repeatability and reproducibility, and acceptable long-term stability. The clinical analysis of the manufactured immunosensor was explored for the analysis of serum samples, and high recoveries and good relative standard deviations (RSDs) were obtained in the range of 97.01 and 103.17 %, respectively. This copolymer-based biosensor could be a feasible tool for clinical diagnosis in complex serum samples.
dc.description.sponsorshipScientific and Technological Research Council of by Tekirdag Namik Kemal University [NKUBAP.00. GA.21.328]
dc.description.sponsorshipThis current study was financially supported by the Scientific and Technological Research Council of by Tekirdag Namik Kemal University (Project No. NKUBAP.00. GA.21.328).
dc.identifier.doi10.1016/j.microc.2024.111464
dc.identifier.issn0026-265X
dc.identifier.issn1095-9149
dc.identifier.scopus2-s2.0-85202482379
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.microc.2024.111464
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14333
dc.identifier.volume206
dc.identifier.wosWOS:001316941800001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofMicrochemical Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectConductive copolymer layer
dc.subjectEarly diagnosis
dc.subjectCYFRA 21-1
dc.subjectLabel-free analysis
dc.titleFabrication of a highly sensitive conductive copolymer layer modified immunosensing tool for cytokeratin-19 fragment detection
dc.typeArticle

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