INVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS

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dc.contributor.authorYüksel, Tuğba Nurcan
dc.contributor.authorYayla, Muhammed
dc.contributor.authorKöse, Duygu
dc.contributor.authorUgan, Rüstem Anıl
dc.contributor.authorTokay, Erdem
dc.contributor.authorKılıcle, Pınar Aksu
dc.contributor.authorÇadırcı, Elif
dc.date.accessioned2024-10-29T17:50:23Z
dc.date.available2024-10-29T17:50:23Z
dc.date.issued2023
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractPomegranate peel contains bioactive ingredients such as flavonoids, ellagitannins, phenolics and proanthocyanidin compounds with high antioxidant activity. Pomegranate peel has antiapoptotic, antioxidant and anti-inflammatory effects due to its high punicalagin content. We aimed to determine the effect of pomegranate peel extract (PPE) on lipopolysaccharide (LPS)-induced uveitis. Sixty rats were seperated randomly into twelve groups (n = 5). The healthy group received intraperitoneal normal saline, the uveitis group received 200 ?g/kg LPS, the dexamethasone (DEX) group received 200 ?g/kg LPS plus 1 mg/kg DEX, the PPE100, PPE300 and PPE500 groups received 200 ?g/kg LPS plus 100, 300 and 500 mg/kg PPE, respectively. The eye tissues were collected at 3rd and 24th hour. and investigated molecularly (Relative quantification of gene expression), biochemically (Superoxide dismutase activity, Glutathione and Malondialdehyde levels) and histopathologically (staining with Harris Hematoxylin and Eosin Y). Tumor Necrosis Factor-?, vascular endothelial growth factor, and Caspase-3 levels markedly decreased in a dose-dependent manner in the uveitic rats following PPE administration. PPE administration significantly ameliorated uveitic disorders in oxidative stress factors including Glutathione, Superoxide dismutase and Malondialdehyde, with its effects raising in a dose-dependent manner. PPE eliminated histopathological changes in eye tissues due to uveitis. PPE can be a promising agent by contributing to alternative preventive treatment methods for uveitis with its anti-inflammatory, antioxidative, antiapoptotic and antiangiogenic effects.
dc.identifier.doi10.23902/trkjnat.1145462
dc.identifier.endpage20
dc.identifier.issn2147-0294
dc.identifier.issn2528-9691
dc.identifier.issue1en_US
dc.identifier.startpage11
dc.identifier.trdizinid1164023
dc.identifier.urihttps://doi.org/10.23902/trkjnat.1145462
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1164023
dc.identifier.urihttps://hdl.handle.net/20.500.11776/12966
dc.identifier.volume24
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofTrakya University Journal of Natural Sciences
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTNF-?
dc.subjectAntioxidant
dc.subjectAnti-inflammatory
dc.subjectPomegranate peel
dc.subjectLPS-induced uveitis
dc.subjectPunicalagin
dc.titleINVESTIGATION OF THE PROTECTIVE EFFECTS OF POMEGRANATE (Punica granatum L.) PEEL EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED UVEITIS IN RATS
dc.typeArticle

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