Highly selective and sensitive sandwich immunosensor platform modified with MUA-capped GNPs for detection of spike Receptor Binding Domain protein: A precious marker of COVID-19 infection

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dc.authorscopusid57194273220
dc.authorscopusid55571487900
dc.authorscopusid6506764918
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorAydın, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:07:25Z
dc.date.available2022-05-11T14:07:25Z
dc.date.issued2021
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractA label-free electrochemical biosensing system as a suitable analysis technique for COVID 19 specific spike receptor-binding domain protein (RBD) was developed with an aim to facilitate the diagnosis of coronavirus. A novel production procedure for the fabrication of gold nanoparticles (GNPs)-capped 11-mercaptoundecanoic acid (MUA) modified bioelectrode was presented and its application potential for RBD biosensing was examined. The bioelectrode fabrication protocol was based on covalent ester linking formation between hydroxylated ITO electrode and GNPs-capped MUA (GNPs@MUA) with carboxyl ends. For this aim, spherical GNPs were prepared and characterized with scanning-transmission electron microscopy (S-TEM), UV–vis, and Raman spectroscopy. The synthesized GNPs were functionalized with MUA yielding Au-S bonds. Then, covalent immobilization of anti-RBD antibodies on the GNPs@MUA was performed with the help of carbodiimide coupling chemistry. The assembly processes of GNPs@MUA, anti-RBD antibodies and RBD antigens were characterized electrochemical, chemical and morphological techniques. GNPs@MUA was used as immobilization environment and provided the most effective surface design for target immunosensor. The resulting immunosensor is further applied to the impedimetric detection of RBD and it displayed a linear response to RBD antigen in the linear range of 0.002?100 pg mL?1 with a limit of detection of 0.577 fg mL?1 and sensitivity of 0.238 kohmpgmL?1 cm?2. The fabricated immunosensor had a good repeatability, long storage, stability and a reusable property after simple regeneration process. Furthermore, it was successfully employed for selective determination of RBD in artificial nasal secretion samples. © 2021
dc.description.sponsorshipFIA-2020-3304
dc.description.sponsorshipThe authors would like to acknowledge the financial support from The Scientific and Technological Research Council of ?anakkale Onsekiz Mart University, (FIA-2020-3304).
dc.description.sponsorshipThe authors would like to acknowledge the financial support from The Scientific and Technological Research Council of Çanakkale Onsekiz Mart University , ( FIA-2020-3304 ).
dc.identifier.doi10.1016/j.snb.2021.130355
dc.identifier.issn0925-4005
dc.identifier.scopus2-s2.0-85109199227
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.snb.2021.130355
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5102
dc.identifier.volume345
dc.identifier.wosWOS:000685511100006
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorAydın, Elif Burcu
dc.institutionauthorAydın, Muhammet
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofSensors and Actuators, B: Chemical
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMUA-capped gold nanoparticles
dc.subjectSandwich immunosensor
dc.subjectSingle-use biosensor
dc.subjectSpike receptor binding domain
dc.subjectAntibodies
dc.subjectAntigens
dc.subjectComputer software reusability
dc.subjectFiber optic sensors
dc.subjectGold compounds
dc.subjectGold nanoparticles
dc.subjectHigh resolution transmission electron microscopy
dc.subjectImmunosensors
dc.subjectScanning electron microscopy
dc.subject11-mercaptoundecanoic acid
dc.subject11-mercaptoundecanoic acid-capped gold nanoparticle
dc.subjectBio electrodes
dc.subjectGold nano-particles
dc.subjectGold nanoparticle
dc.subjectProtein antigens
dc.subjectReceptor-binding domains
dc.subjectSandwich immunosensor
dc.subjectSingle-use biosensor
dc.subjectSpike receptor binding domain
dc.subjectMetal nanoparticles
dc.titleHighly selective and sensitive sandwich immunosensor platform modified with MUA-capped GNPs for detection of spike Receptor Binding Domain protein: A precious marker of COVID-19 infection
dc.typeArticle

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