Enhanced E2F1 activity increases invasive and proliferative activity of breast cancer cells through non-coding RNA CDKN2B-AS1

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dc.authorscopusid56497061100
dc.authorscopusid55815636400
dc.authorscopusid56190917500
dc.authorscopusid36772919600
dc.authorscopusid35482179000
dc.contributor.authorBozgeyik, Esra
dc.contributor.authorSaadat, Khandakar A.S.M.
dc.contributor.authorArman, K.
dc.contributor.authorBozgeyik, İbrahim
dc.contributor.authorİkeda, M.A.
dc.date.accessioned2022-05-11T14:05:12Z
dc.date.available2022-05-11T14:05:12Z
dc.date.issued2020
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalı
dc.description.abstractLong non-coding RNAs have recently appeared as fundamental regulators of gene transcription in several biological processes, but only a few have known functional influences in the malignant transformation of breast cancer. CDKN2B-AS1 gene, also termed ANRIL, encoding a long non-coding RNA is located in the CDKN2B-CDKN2A gene cluster, loss of which is the most frequent alternation in several types of human malignancies. CDKN2B-AS1 is involved in the suppression of tumor suppressor genes (INK4a, ARF, and INK4b) and has been recognized as a direct target of E2F1. However, the roles of E2F1–CDKN2B-AS1 interaction in breast cancer have remained muchly mysterious. In this particular study, we reveal that both CDKN2B and CDKN2B-AS1 genes were differentially expressed in breast cancer cells in contrast to breast epithelial cells. Ectopic expression of E2F1 activated CDKN2B-AS1 but not CDKN2B expression. Lastly, overexpression of E2F1 improved the colony formation and migratory capacities of breast cancer cells. These results suggest that enhanced E2F1 activity increased invasive and proliferative activity of breast cancer but not breast epithelial cells possibly through up-regulating CDKN2B-AS1 transcript. © 2020 Elsevier B.V.
dc.identifier.doi10.1016/j.mgene.2020.100691
dc.identifier.issn2214-5400
dc.identifier.scopus2-s2.0-85082201284
dc.identifier.scopusqualityQ4
dc.identifier.urihttps://doi.org/10.1016/j.mgene.2020.100691
dc.identifier.urihttps://hdl.handle.net/20.500.11776/4918
dc.identifier.volume24
dc.identifier.wosWOS:000525750700025
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorBozgeyik, Esra
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofMeta Gene
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBreast Cancer
dc.subjectCDKN2B-AS1
dc.subjectE2F1
dc.subjectNatural antisense transcript
dc.subjectcomplementary DNA
dc.subjectcomplementary RNA
dc.subjectcyclin dependent kinase inhibitor 2A
dc.subjectcyclin dependent kinase inhibitor 2B
dc.subjectcyclin dependent kinase inhibitor 2b antisense RNA 1
dc.subjectglyceraldehyde 3 phosphate dehydrogenase
dc.subjecttranscription factor E2F1
dc.subjectunclassified drug
dc.subjectuntranslated RNA
dc.subjectARF gene
dc.subjectArticle
dc.subjectbinding site
dc.subjectbioinformatics
dc.subjectbreast cancer
dc.subjectbreast cancer cell line
dc.subjectbreast epithelium cell
dc.subjectcarcinogenesis
dc.subjectCDKN2B AS1 gene
dc.subjectCDKN2B gene
dc.subjectcell invasion
dc.subjectcell migration
dc.subjectcell proliferation
dc.subjectcolony forming unit
dc.subjectcontrolled study
dc.subjectCRL4010 cell line
dc.subjectdown regulation
dc.subjectgene
dc.subjectgene expression
dc.subjectgene expression level
dc.subjectgene overexpression
dc.subjectgenetic transcription
dc.subjectgenetic transfection
dc.subjecthuman
dc.subjecthuman cell
dc.subjectINK4a gene
dc.subjectINK4b gene
dc.subjectMCF-7 cell line
dc.subjectMDA-MB-231 cell line
dc.subjectpriority journal
dc.subjectpromoter region
dc.subjectprotein expression
dc.subjectreal time reverse transcription polymerase chain reaction
dc.subjectSK-BR-3 cell line
dc.subjecttumor suppressor gene
dc.subjectupregulation
dc.subjectwound healing assay
dc.titleEnhanced E2F1 activity increases invasive and proliferative activity of breast cancer cells through non-coding RNA CDKN2B-AS1
dc.typeArticle

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