Silencing of TP73-AS1 impairs prostate cancer cell proliferation and induces apoptosis via regulation of TP73

Basit Öğe Kaydı
dc.authorscopusid56468900300
dc.authorscopusid15764510600
dc.authorscopusid56221906000
dc.authorscopusid6504396778
dc.authorscopusid57203094952
dc.authorscopusid56497061100
dc.contributor.authorArslan, A.
dc.contributor.authorBatar, Bahadır
dc.contributor.authorTemiz, E.
dc.contributor.authorTozkır, Hilmi
dc.contributor.authorKoyuncu, İsmail
dc.contributor.authorBozgeyik, Esra
dc.date.accessioned2022-05-11T14:02:44Z
dc.date.available2022-05-11T14:02:44Z
dc.date.issued2022
dc.departmentFakülteler, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Genetik Ana Bilim Dalı
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalı
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.description.abstractBackground: Prostate cancer is a malignant disease that severely affects the health and comfort of the male population. The long non-coding RNA TP73-AS1 has been shown to be involved in the malignant transformation of various human cancers. However, whether TP73-AS1 contributes to prostate cancer progression has not been reported yet. Accordingly, here we aimed to report the role of TP73-AS1 in the development and progression of prostate cancer and determine its relationship with TP73. Methods and results: TP73-AS1-specific siRNA oligo duplexes were used to silence TP73-AS1 in DU-145 and PC-3 cells. Results indicated that TP73-AS1 was upregulated whereas TP73 was downregulated in prostate cancer cells compared to normal prostate cells and there was a negative correlation between them. Besides, loss of function experiments of TP73-AS1 in prostate cancer cells strongly induced cellular apoptosis, interfered with the cell cycle progression, and modulated related pro- and anti-apoptotic gene expression. Colony formation and migration capacities of TP73-AS1-silenced prostate cancer cells were also found to be dramatically reduced. Conclusions: Our findings provide novel evidence that suggests a chief regulatory role for the TP73-TP73-AS1 axis in prostate cancer development and progression, suggesting that the TP73/TP73-AS1 axis can be a promising diagnostic and therapeutic target for prostate cancer. © 2022, The Author(s), under exclusive licence to Springer Nature B.V.
dc.description.sponsorshipONAP.19.205
dc.description.sponsorshipThis study was supported by Tekirdag Namik Kemal University (Grant no. NKUBAP.02.ONAP.19.205).
dc.identifier.doi10.1007/s11033-022-07141-6
dc.identifier.issn0301-4851
dc.identifier.pmid35138524
dc.identifier.scopus2-s2.0-85124550767
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1007/s11033-022-07141-6
dc.identifier.urihttps://hdl.handle.net/20.500.11776/4459
dc.identifier.wosWOS:000753244700002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorArslan, A.
dc.institutionauthorBatar, Bahadır
dc.institutionauthorTozkır, Hilmi
dc.language.isoen
dc.publisherSpringer Science and Business Media B.V.
dc.relation.ispartofMolecular Biology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectLncRNA
dc.subjectProstate cancer
dc.subjectTP73
dc.subjectTP73-AS1
dc.titleSilencing of TP73-AS1 impairs prostate cancer cell proliferation and induces apoptosis via regulation of TP73
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
4459.pdf
Boyut:
4.35 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Tıp Fakültesi Koleksiyonu