Long noncoding RNA ERICD interacts with ARID3A via E2F1 and regulates migration and proliferation of osteosarcoma cells

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Küçük Resim

Tarih

2020

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Blackwell Publishing Ltd

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Long noncoding RNA (lncRNA) dysregulation is known to be taking part in majority of cancers, including osteosarcoma. In one of our previous studies, we showed that lncRNA MEG3 is being regulated by microRNA-664a (miR-664a) suppresses the migratory potential of osteosarcoma cells (U-2OS). We now report a novel lncRNA, namely, ERICD, which is linked to the transcription factor AT-rich interaction domain 3A (ARID3A) in U-2OS cells. We show that ARID3A binds to ERICD and indirectly interacts with each other via the E2F transcription factor 1 (E2F1). Furthermore, small interfering RNA (siRNA)-mediated knockdown of ERICD inhibited cell migration, formation of colonies, and proliferation in U-2OS cells. Overexpression of ARID3A inhibited cell migration, colony formation, and proliferation, whereas siRNA-mediated knockdown of ARID3A promoted cell migration, colony formation, and proliferation. Our findings indicate that ARID3A and lncRNA ERICD have plausible tumor suppressive and oncogenic functions, respectively, in osteosarcoma. Our data demonstrate the converse interaction between ARID3A and lncRNA ERICD that target DNA-binding proteins and dysregulation of their expression through E2F1 augments osteosarcoma progression. The cell rescue experiment also indicated E2F1 to be involved in the regulation of ARID3A and ERICD. © 2020 International Federation for Cell Biology

Açıklama

Anahtar Kelimeler

ARID3A, colony formation, DNA-binding lncRNA, lncRNA ERICD, migration, osteosarcoma, DNA binding protein, E2F transcription factor 1, long noncoding RNA E2F1 regulated inhibitor of cell death, long untranslated RNA, transcription factor, transcription factor AT rich interaction domain 3A, transcription factor E2F, unclassified drug, ARID3A protein, human, DNA binding protein, E2F1 protein, human, long untranslated RNA, transcription factor, transcription factor E2F1, Article, binding site, cancer growth, cell migration, cell proliferation, cell viability, colony formation, controlled study, down regulation, gene expression regulation, gene function, genetic correlation, human, human cell, osteosarcoma, promoter region, protein expression, protein function, protein RNA binding, regulatory mechanism, U2OS cell line, upregulation, apoptosis, bone tumor, cell motion, cell proliferation, genetics, metabolism, osteosarcoma, pathology, tumor cell line, Apoptosis, Bone Neoplasms, Cell Line, Tumor, Cell Movement, Cell Proliferation, DNA-Binding Proteins, E2F1 Transcription Factor, Gene Expression Regulation, Neoplastic, Humans, Osteosarcoma, RNA, Long Noncoding, Transcription Factors

Kaynak

Cell Biology International

WoS Q Değeri

Q3

Scopus Q Değeri

Q1

Cilt

44

Sayı

11

Künye