Avokado/soya fasülyesinin sabunlaşmayan ekstresi verilen köpeklerin dizlerinde osteokondral defekt iyileşmesi: Bir karşılaştırmalı morfometrik analiz
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Tarih
2011
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info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada avocado/soya fasülyesinin sabunlaşmayan ekstresinin (ASE) köpek osteokondral defekt modelinde iyileşmeye etkisi belirlendi. Gereç ve yöntemler: On dört köpek çalışmaya alındı ve rastgele olarak iki gruba ayrıldı. Diz ekleminin troklear oluğunun lateral yüzünde iki adet osteokondral defekt oluşturuldu. Tedavi grubuna (grup 1; n=7) her üç günde bir 300 mg’lik ASE kapsülleri, kontrol grubuna (grup 2; n=7) ise normal diyet verildi. On beş hafta sonrasında ötenazi yapılana kadar hayvanlar kafesler içinde serbest gezinmeye bırakıldı. Dizler açılarak defektli troklear oluklar patolojik inceleme için çıkarıldı. Rejenere doku miktarı görüntü analiz yöntemiyle kantitatif olarak, doku içeriği ise Safranin-O ve Masson trikrom histokimyasal boyamaları ile semikantitatif olarak değerlendirildi. Transforme edici büyüme faktörü-beta (TGF-b) artışı, immünhistokimyasal boyama yöntemiyle semikantitatif olarak değerlendirildi. Bulgular: Morfometrik analiz sonucunda grup 1’de anlamlı ölçüde daha fazla immatür iyileşme dokusu saptandı (p<0.002). Grup 1’de rejenere dokuda hem kollajen hem de kondral doku içeriği anlamlı ölçüde artmıştı (p<0.002). Grup 2’ye kıyasla grup 1’de kıkırdak dokusunda TGF-b immün boyanma reaksiyonu çok daha belirgindi. Sonuç: Avocado/soya fasülyesinin sabunlaşmayan ekstresi ile yapılan tedavi, köpek dizinde osteokondral defekt iyileşmesini uyarmakta ve muhtemelen dokuda TGF-b’yı artırarak bu etkiyi sağlamaktadır.
Objectives: This study aims to determine the effects of avocado/ soybean unsaponifiables (ASU) on healing in a canine osteochondral defect model. Materials and methods: Fourteen dogs were included in the study and randomly divided into two groups. Two osteochondral defects were produced in the lateral aspect of the trochlear groove of the knee joint. The treatment group (group 1; n=7) was given 300 mg ASU capsules every three days whereas the control group (group 2; n=7) was given a normal diet. Animals were then allowed to ambulate normally until euthanasia at 15 weeks. The knees were dissected and the trochlear grooves with defects were removed for pathological examination. The amount of regenerated tissue was determined quantitatively using image analysis and the tissue content was evaluated semi-quantitatively using Safranin-O and Masson trichrome histochemical stains. Transforming growth factor beta (TGF-b) increase was evaluated semiquantitatively with immunohistochemical staining methods. Results: Morphometric analysis revealed a significantly more immature repair tissue in group 1 (p<0.002). Both collagen and chondral tissue content of the regenerated tissue were significantly increased in group 1 (p<0.002). Compared to that in group 2, cartilage tissue in group 1 showed a much more marked immunostaining reaction of TGF-b. Conclusion: Avocado/soybean unsaponifiables treatment stimulates t he healing of t he osteochondral d efects in canine knee possibly by increasing TGF-b i n t he t issues.
Objectives: This study aims to determine the effects of avocado/ soybean unsaponifiables (ASU) on healing in a canine osteochondral defect model. Materials and methods: Fourteen dogs were included in the study and randomly divided into two groups. Two osteochondral defects were produced in the lateral aspect of the trochlear groove of the knee joint. The treatment group (group 1; n=7) was given 300 mg ASU capsules every three days whereas the control group (group 2; n=7) was given a normal diet. Animals were then allowed to ambulate normally until euthanasia at 15 weeks. The knees were dissected and the trochlear grooves with defects were removed for pathological examination. The amount of regenerated tissue was determined quantitatively using image analysis and the tissue content was evaluated semi-quantitatively using Safranin-O and Masson trichrome histochemical stains. Transforming growth factor beta (TGF-b) increase was evaluated semiquantitatively with immunohistochemical staining methods. Results: Morphometric analysis revealed a significantly more immature repair tissue in group 1 (p<0.002). Both collagen and chondral tissue content of the regenerated tissue were significantly increased in group 1 (p<0.002). Compared to that in group 2, cartilage tissue in group 1 showed a much more marked immunostaining reaction of TGF-b. Conclusion: Avocado/soybean unsaponifiables treatment stimulates t he healing of t he osteochondral d efects in canine knee possibly by increasing TGF-b i n t he t issues.
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Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.)
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Cilt
22
Sayı
1
