Cryopreservation of adenovirus-transfected dendritic cells (DCs) for clinical use

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dc.authorid0000-0002-7792-7459
dc.authorid0000-0002-6328-6056
dc.authorid0000-0002-8711-7820
dc.authorscopusid6602366586
dc.authorscopusid25632466600
dc.authorscopusid24463483600
dc.authorscopusid7006336302
dc.authorscopusid55173255200
dc.authorscopusid35175256600
dc.authorscopusid23493641400
dc.authorwosidGulen, Dumrul/A-5955-2017
dc.authorwosidYounos, Ibrahim H/F-9225-2015
dc.authorwosidTalmadge, James/AAF-7242-2020
dc.contributor.authorGülen, Dumrul
dc.contributor.authorMaas, S.
dc.contributor.authorJulius, H.
dc.contributor.authorWarkentin, P.
dc.contributor.authorBritton, H.
dc.contributor.authorYounos, İbrahim H.
dc.contributor.authorTalmadge, J. E.
dc.date.accessioned2022-05-11T14:42:10Z
dc.date.available2022-05-11T14:42:10Z
dc.date.issued2012
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalı
dc.description.abstractIn this study, we examined the effects of cryoprotectant, freezing and thawing, and adenovirus (Adv) transduction on the viability, transgene expression, phenotype, and function of human dendritic cells (DCs). DCs were differentiated from cultured peripheral blood (PB) monocytes following Elutra isolation using granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) for 6 days and then transduced using an Adv vector with an IL-12 transgene. Fresh, cryopreserved, and thawed transduced immature DCs were examined for their: 1) cellular concentration and viability; 2) antigenicity using an allogeneic mixed lymphocyte reaction (MLR); 3) phenotype (HLA-DR and CD11c) and activation (CD83); and 4) transgene expression based on IL-12 secretion. Stability studies revealed that transduced DCs could be held in cryoprotectant for as long as 75 min at 2-8 degrees C prior to freezing with little effect on their viability and cellularity. Further, cryopreservation, storage, and thawing reduced the viability of the transduced DCs by an average of 7.7%; and had no significant impact on DC phenotype and activation. In summary, cryopreservation, storage, and thawing had no significant effect on DC viability, function, and transgene expression by Adv-transduced DCs. (C) 2012 Elsevier B.V. All rights reserved.
dc.description.sponsorshipNebraska Research Initiative
dc.description.sponsorshipThis research was funded through the Nebraska Research Initiative. None of the contributing authors has any financial conflict of interest.
dc.identifier.doi10.1016/j.intimp.2012.03.009
dc.identifier.endpage68
dc.identifier.issn1567-5769
dc.identifier.issue1en_US
dc.identifier.pmid22465385
dc.identifier.scopus2-s2.0-84859339453
dc.identifier.scopusqualityQ1
dc.identifier.startpage61
dc.identifier.urihttps://doi.org/10.1016/j.intimp.2012.03.009
dc.identifier.urihttps://hdl.handle.net/20.500.11776/9258
dc.identifier.volume13
dc.identifier.wosWOS:000304580200009
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorGülen, Dumrul
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofInternational Immunopharmacology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCryopreservation
dc.subjectDendritic cells
dc.subjectAdenovirus
dc.subjectStability
dc.subjectViability
dc.subjectMessenger-Rna
dc.subjectRecombinant Adenovirus
dc.subjectMalignant-Melanoma
dc.subjectProstate-Cancer
dc.subjectVaccination
dc.subjectAntigen
dc.subjectGeneration
dc.subjectEfficient
dc.subjectP53
dc.subjectImmunotherapy
dc.titleCryopreservation of adenovirus-transfected dendritic cells (DCs) for clinical use
dc.typeArticle

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