High-throughput sequencing of maize dwarf mosaic virus from common reed in a wetland

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dc.authorid, Rick/0000-0002-6848-2144
dc.contributor.authorIlbagi, Havva
dc.contributor.authorMasonbrink, Rick
dc.contributor.authorMiller, W. Allen
dc.date.accessioned2024-10-29T17:58:45Z
dc.date.available2024-10-29T17:58:45Z
dc.date.issued2023
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractCommon reed is a widespread aquatic grass in wetland areas of Turkey. In reed samples, RNAs with the sequence of the Maize dwarf mosaic virus (MDMV) were found using high- throughput sequencing. The nearly complete genome sequence comprised 9514 nucleotides. It contains a 5' UTR of at least 145 nucleotides, a 3' UTR of at least 237 nucleotides, and an ORF encoding a single polyprotein of 3041 amino acid residues. Two partial sequences were 7709 nt and 7858 nt long, respectively. Three reed sequences contained 22 nt, 25 nt, and 80 nt insertions in the 5'UTR, respectively. The coding region of MDMV TR- 34 (49%) was more conserved than UTRs (34.3%) compared to Johnsongrass mosaic virus (JGMV), Sugarcane mosaic virus (SCMV), and Sorghum mosaic virus (SrMV). MDMV- TR34 reed isolate shares 91%- 53.1% nucleotide identity and 98.2%- 49% amino acid sequence similarity with other MDMV and three potyvirus sequences, respectively. Recombination analysis indicated six putative re -combination events. In 2020- 2022, the survey studies showed that 25 of 260 reed samples were infected with MDMV at the infection rate of 9.6% in Tekirdag province. This result is the first report of the complete nucleotidesequence of MDMV in the common reed, a reservoir host for cereals growing areas in Turkey.
dc.description.sponsorshipTekirdag Namik Kemal University, The Scientific Research Projects Coordination Unit (NKU-BAP) [NKUBAP.03.GA.21.289]; Iowa Agriculture and Home Economics Experiment Station, Ames, IA - Hatch Act [4308]; Iowa Agriculture and Home Economics Experiment Station, Ames, IA - State of Iowa funds [4308]; Iowa State University Plant Sciences Institute; DARPA Insect Allies Program; Scientific and Technological Research Council of Turkey, International Postdoctoral Research Fellowship Program (TUBITAK-BIDEB)
dc.description.sponsorshipTekirdag Namik Kemal University, The Scientific Research Projects Coordination Unit (NKU-BAP, Project No: NKUBAP.03.GA.21.289) to H.I.; The Iowa Agriculture and Home Economics Experiment Station, Ames, IA, Project No. 4308 was supported in part by Hatch Act and State of Iowa funds to W.A.M.; The Iowa State University Plant Sciences Institute and the DARPA Insect Allies Program funding to W.A.M.; The Scientific and Technological Research Council of Turkey, International Postdoctoral Research Fellowship Program (TUBITAK-BIDEB) to H.I.
dc.identifier.doi10.1111/jph.13216
dc.identifier.endpage608
dc.identifier.issn0931-1785
dc.identifier.issn1439-0434
dc.identifier.issue45637en_US
dc.identifier.scopus2-s2.0-85168349422
dc.identifier.scopusqualityQ2
dc.identifier.startpage604
dc.identifier.urihttps://doi.org/10.1111/jph.13216
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14484
dc.identifier.volume171
dc.identifier.wosWOS:001049955300001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Phytopathology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjecthigh-throughput sequencing
dc.subjectMDMV
dc.subjectrecombination analysis
dc.subjectreed
dc.titleHigh-throughput sequencing of maize dwarf mosaic virus from common reed in a wetland
dc.typeArticle

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