Salmonella carriage and change in serovar distribution in broiler giblets at slaughterhouse level in Turkiye: first report using ISO 6579-1:2017 and ISO 6579-3:2014

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dc.contributor.authorAkgun, Z.
dc.contributor.authorCoskun, A. G.
dc.contributor.authorCetin, E.
dc.contributor.authorTemelli, S.
dc.contributor.authorEyigor, A.
dc.date.accessioned2024-10-29T17:58:28Z
dc.date.available2024-10-29T17:58:28Z
dc.date.issued2024
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractThis study aimed to determine the prevalence and serovar distribution of salmonellae in liver, heart, and spleen ( LHS ) and gizzard ( G ) of slaughtered broilers. For this, a total of 60 sample units, comprised of 30 LHS and 30 G collected from 3 slaughterhouses, were analysed by reference methods for detection and serotyping as revised ISO 6579-1:2017 and ISO 6579-3:2014, respectively. Also, Salmonellaspecific real-time PCR ( Salm-PCR ) was used for species confirmation, while Salmonella Enteritidis ( S . Enteritidis ) and Salmonella Typhimurium ( S . Typhimurium ) specific real-time PCR (SE/ST-PCR) was evaluated to determine its efficiency for rapid detection of the serovars mandated in current legal regulations compared to standard serotyping. All LHS (100% -30/30) and 90% (27/30) of G samples harbored Salmonella with an overall prevalence of 95% (57/60) in samples examined, where all isolates were confirmed as Salmonella by Salm-PCR. The most prevalent serovar in broiler giblets was S. Virchow (80.70% -46/57) followed by S. Enteritidis (19.30% -11/57). SE/STPCR (%17.54 -10/57) could not detect one G isolate, which was serotyped as S. Enteritidis by standard serotyping. High relative accuracy (98.25%), sensitivity (100%) and specificity (100%), and agreement between methods ( K : 0.94) verified SE/ST-PCR 's potential to be used as an alternative in rapid detection of S . Enteritidis and S . Typhimurium. Data on high Salmonella prevalence in broiler giblets of slaughterhouse origin, and detection of the pathogen by the implementation of all requirements indicated in the revised ISO 65791:2017 standard method, enabling the determination of actual prevalence in the samples with high sensitivity and specificity is of significance for public health. Additionally, identification of S . Virchow as the dominant serovar followed by S . Enteritidis with a relatively lower prevalence, and absence of S. Typhimurium in broiler giblets are important findings for Turkiye. This up to date data, obtained by strict application of ISO 6579-3:2014 procedures, indicated a shift in circulating serovars in the broiler industry. The objective findings in this study would bring awareness to national/international literature, and may be of use in future improvements in legal regulations.
dc.description.sponsorshipBursa Uludag University Scientific Research Unit Grant [TGA-2021-488]
dc.description.sponsorshipFunding for this project was provided by the Bursa Uludag University Scientific Research Unit Grant, Project No: TGA-2021-488. The authors thank the veterinarians and the personnel in all slaughterhouses for their technical support during sample handling and transport.
dc.identifier.doi10.1016/j.psj.2024.103805
dc.identifier.issn0032-5791
dc.identifier.issn1525-3171
dc.identifier.issue7en_US
dc.identifier.pmid38749106
dc.identifier.scopus2-s2.0-85192799010
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.psj.2024.103805
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14339
dc.identifier.volume103
dc.identifier.wosWOS:001242701900001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofPoultry Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSalmonella
dc.subjectbroiler
dc.subjectgiblet
dc.subjectISO 6579-1:2017
dc.subjectISO 6579-3:2014
dc.subjectreal-time PCR
dc.titleSalmonella carriage and change in serovar distribution in broiler giblets at slaughterhouse level in Turkiye: first report using ISO 6579-1:2017 and ISO 6579-3:2014
dc.typeArticle

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