Highly sensitive impedimetric immunosensor for determination of interleukin 6 as a cancer biomarker by using conjugated polymer containing epoxy side groups modified disposable ITO electrode

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dc.authorscopusid57194273220
dc.contributor.authorAydın, Elif Burcu
dc.date.accessioned2022-05-11T14:07:24Z
dc.date.available2022-05-11T14:07:24Z
dc.date.issued2020
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractA novel impedimetric immunosensor based on a conjugated polypyrrole polymer containing epoxy active side groups (PPCE) modified indium tin oxide (ITO) electrode was developed for detection of interleukin 6 (IL 6), a prostate cancer biomarker. IL 6 receptor was used as a biorecognition molecule and successfully immobilized by covalent linkage on the modified ITO electrode. Pyrrole monomer containing epoxy active side group was electropolymerized on the disposable ITO electrode for the first time and used as an immobilization matrix for IL 6 receptor. The designed biosensor fabrication stages were analyzed by using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. For the quantification of IL 6 biomarker, EIS technique was utilized. In addition, the specific immuno-interaction between IL 6 receptor and IL 6 antigen was followed by single frequency impedance (SFI) technique. The resulting biosensor exhibited a linear calibration curve over the 0.02–16 pg/mL IL 6 concentration range, a limit of detection (LOD) of 6.0 fg/mL and a good selectivity against other interference biomarkers. Additionally, an excellent reproducibility, a long storage stability and an acceptable repeatability were found. The proposed impedimetric immunosensor was applied successfully to quantify for the IL 6 biomarker in human serum and it displayed a remarkable response in the real sample analysis with serum samples. In comparison with the commercial ELISA kit, the immunosensor provided a lower LOD and a lower analysis cost. In conclusion, the proposed impedimetric immunosensor could be clinically useful in the early diagnosis of the prostate cancer by detection of the serum samples after only simple dilution with phosphate buffer. © 2020 Elsevier B.V.
dc.identifier.doi10.1016/j.talanta.2020.120909
dc.identifier.issn0039-9140
dc.identifier.pmid32312453
dc.identifier.scopus2-s2.0-85081679297
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2020.120909
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5095
dc.identifier.volume215
dc.identifier.wosWOS:000528209200023
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Elif Burcu
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofTalanta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectImpedimetric immunosensor
dc.subjectInterleukin 6
dc.subjectProstate cancer
dc.subjectPyrrole-based conjugated polymer containing epoxy active side groups
dc.subjectAromatic compounds
dc.subjectConjugated polymers
dc.subjectCyclic voltammetry
dc.subjectDiagnosis
dc.subjectDiseases
dc.subjectElectrochemical impedance spectroscopy
dc.subjectElectrodes
dc.subjectImmunosensors
dc.subjectPolypyrroles
dc.subjectTin oxides
dc.subjectUrology
dc.subjectImmobilization matrices
dc.subjectImpedimetric immunosensors
dc.subjectIndium tin oxide electrodes
dc.subjectInterleukin-6
dc.subjectLinear calibration curve
dc.subjectModified ito electrodes
dc.subjectProstate cancers
dc.subjectSide groups
dc.subjectBiomarkers
dc.subjectepoxide
dc.subjectindium tin oxide
dc.subjectinterleukin 6
dc.subjectpolymer
dc.subjectpolypyrrole
dc.subjectpyrrole derivative
dc.subjecttin derivative
dc.subjecttumor marker
dc.subjectchemistry
dc.subjectelectrochemical analysis
dc.subjectelectrode
dc.subjectgenetic procedures
dc.subjecthuman
dc.subjectparticle size
dc.subjectsurface property
dc.subjectsynthesis
dc.subjectBiomarkers, Tumor
dc.subjectBiosensing Techniques
dc.subjectElectrochemical Techniques
dc.subjectElectrodes
dc.subjectEpoxy Compounds
dc.subjectHumans
dc.subjectInterleukin-6
dc.subjectParticle Size
dc.subjectPolymers
dc.subjectPyrroles
dc.subjectSurface Properties
dc.subjectTin Compounds
dc.titleHighly sensitive impedimetric immunosensor for determination of interleukin 6 as a cancer biomarker by using conjugated polymer containing epoxy side groups modified disposable ITO electrode
dc.typeArticle

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