Anti-inflammatory and ultrastructural effects of Turkish propolis in a rat model of endotoxin-induced uveitis

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dc.authorid0000-0002-0649-844X
dc.authorid0000-0002-5605-2980
dc.authorid0000-0001-7341-4907
dc.authorid0000-0001-6691-1235
dc.authorscopusid56141881600
dc.authorscopusid57189239841
dc.authorscopusid11440747800
dc.authorscopusid6602548692
dc.authorscopusid7004147590
dc.authorscopusid6602904773
dc.authorscopusid50263136900
dc.authorwosidEKMEKÇİ, HAKAN/AAC-9833-2021
dc.authorwosidGungor, Zeynep/D-3779-2019
dc.authorwosidKeskinbora, Kadircan/AAM-6453-2020
dc.authorwosidekmekci, hakan/D-4969-2019
dc.authorwosidgocmez, semil selcen/G-2401-2018
dc.authorwosidyaprak saraç, elif/L-3752-2016
dc.contributor.authorErturkuner, Salime Pelin
dc.contributor.authorSarac, Elif Yaprak
dc.contributor.authorGöçmez, Semil Selcen
dc.contributor.authorEkmekci, Hakan
dc.contributor.authorÖztürk, Zeynep Banu
dc.contributor.authorSeckin, İsmail
dc.contributor.authorKeskinbora, Kadircan Hıdır
dc.contributor.authorSever, Özkan
dc.date.accessioned2022-05-11T14:12:29Z
dc.date.available2022-05-11T14:12:29Z
dc.date.issued2016
dc.departmentFakülteler, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Farmakoloji Ana Bilim Dalı
dc.departmentFakülteler, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Kulak Burun ve Boğaz Hastalıkları Ana Bilim Dalı
dc.description.abstractIntroduction. Experimental animal models of acute uveitis, an inflammatory eye disease, can be established via endotoxin-induced inflammation. Propolis, a natural substance collected by honeybees from buds and tree exudates, has antioxidant, antibacterial, antiviral, and anti-inflammatory effects. We investigated the effects of propolis, obtained from the Sakarya province of Turkey, on endotoxin-induced uveitis using immunohistochemical, ultrastructural, and biochemical approaches. Material and methods. Male Wistar albino rats (n = 6/group) received intraperitoneal (ip) lipopolysaccharide (LPS) endotoxin (150 mu g/kg) followed by aqueous extract of propolis (50 mg/kg ip) or vehicle; two additional groups received either saline (control) or propolis only. After 24 h, aqueous humor (AH) was collected from both eyes of each animal for analysis of tumor necrosis factor-alpha (TNF-alpha) and hypoxia-inducible factor-1 alpha (HIF-1 alpha). Right eyeballs were paraffin-embedded for immunohistochemical staining of nuclear factor kappa B (NF-kappa B)/p65 and left eyeballs were araldite-embedded for ultrastructural analysis. Results. Treatment of LPS-induced uveitis with propolis significantly reduced ciliary body NF-kappa B/p65 immunoreactivity and AH levels of HIF-1 alpha and TNF-alpha. Ultrastructural analysis showed fewer vacuoles and reduced mitochondrial degeneration in the retinal pigment epithelium, as compared to the uveitis group. The intercellular spaces of the inner nuclear layer and outer limiting membrane were comparable with those of the control group; no polymorphonuclear cells or stasis was observed in intravascular or extravascular spaces. Conclusions. This is the first report demonstrating an anti-inflammatory effect of Turkish propolis in a rat model of LPS-induced acute uveitis, suggesting a therapeutic potential of propolis for the treatment of inflammatory ophthalmic diseases.
dc.description.sponsorshipScientific Research Projects Coordination Unit of Namik Kemal University [NKUBAP.00.20.AR.12.01]; Azize Gumusyazici and Ercument Boztas, in the electron microscopy laboratory, Cerrahpasa Medical School, Istanbul University
dc.description.sponsorshipThis study was supported by The Scientific Research Projects Coordination Unit of Namik Kemal University (Project no: NKUBAP.00.20.AR.12.01) and was conducted in the Histology and Embryology Department of Cerrahpasa Medical School, Istanbul University, Turkey. We would like to acknowledge the support of Azize Gumusyazici and Ercument Boztas, in the electron microscopy laboratory, Cerrahpasa Medical School, Istanbul University. We also appreciate Aksu Vital Chemical Company for providing us the propolis extract.
dc.identifier.doi10.5603/FHC.a2016.0004
dc.identifier.endpage57
dc.identifier.issn0239-8508
dc.identifier.issn1897-5631
dc.identifier.issue1en_US
dc.identifier.pmid27094636
dc.identifier.scopus2-s2.0-84966659259
dc.identifier.scopusqualityQ3
dc.identifier.startpage49
dc.identifier.urihttps://doi.org/10.5603/FHC.a2016.0004
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5575
dc.identifier.volume54
dc.identifier.wosWOS:000376386800006
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorGöçmez, Semil Selcen
dc.institutionauthorSever, Özkan
dc.language.isoen
dc.publisherVia Medica
dc.relation.ispartofFolia Histochemica Et Cytobiologica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectacute uveitis
dc.subjectpropolis
dc.subjectlipopolysaccharide
dc.subjectHIF-1 alpha
dc.subjectTNF-alpha
dc.subjectIHC
dc.subjectelectron microscopy
dc.subjectrat
dc.subjectNf-Kappa-B
dc.subjectPyrrolidine Dithiocarbamate
dc.subjectIn-Vitro
dc.subjectInflammation
dc.subjectHypoxia
dc.subjectExpression
dc.subjectInhibitor
dc.subjectDiseases
dc.subjectVivo
dc.titleAnti-inflammatory and ultrastructural effects of Turkish propolis in a rat model of endotoxin-induced uveitis
dc.typeArticle

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