An ultrasensitive immunosensor based on tri-armed star poly(glycidyl methacrylate) polymer-coated ITO-PET electrode for detection of neuron-specific enolase in human serum

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dc.authorscopusid55571487900
dc.contributor.authorAydın, M.
dc.date.accessioned2022-05-11T14:07:23Z
dc.date.available2022-05-11T14:07:23Z
dc.date.issued2020
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractIn the present study, an ultrasensitive and simple impedimetric biosensor was fabricated for the determination of neuron-specific enolase (NSE) cancer biomarker. Anti-NSE antibody was covalently immobilised on the indium tin oxide-polyethylene terephthalate (ITO-PET) electrode surface coated with polymer Str(PGMA)3. The star poly(glycidyl methacrylate) polymer Str(PGMA)3 had epoxy side groups and these epoxy groups provided a lot of immobilisation points for antibodies. The process of immunosensor fabrication and successful immobilisation of the antibodies and antigens on the ITO-PET electrode was followed by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. In addition, EIS technique was utilised for the NSE antigen quantification under optimal experimental conditions. NSE concentration was determined in the range from 0.03 pg/mL to 6 pg/mL with an ultralow detection limit of 9.1 fg/mL. Moreover, the surface topology of the modified surfaces was characterised by scanning electron microscopy (SEM) and atomic force microscopy (AFM) imaging. A low detection limit, a good reproducibility, an excellent storage stability and a good selectivity were obtained. This designed biosensor was employed for the determination of NSE antigen level in human serum samples. The obtained results illustrated that this modification procedure could be applied to determine other biomarkers in human serum samples. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
dc.identifier.doi10.1080/03067319.2019.1673744
dc.identifier.endpage506
dc.identifier.issn0306-7319
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85074362199
dc.identifier.scopusqualityQ3
dc.identifier.startpage492
dc.identifier.urihttps://doi.org/10.1080/03067319.2019.1673744
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5092
dc.identifier.volume100
dc.identifier.wosWOS:000515547900011
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorAydın, Muhammet
dc.language.isoen
dc.publisherTaylor and Francis Ltd.
dc.relation.ispartofInternational Journal of Environmental Analytical Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectelectrochemical impedance spectroscopy
dc.subjectlabel free immunosensor
dc.subjectNeuron specific enolase
dc.subjecttri- armed star-polymer
dc.subjectAcrylic monomers
dc.subjectAntibodies
dc.subjectAntigens
dc.subjectAtomic force microscopy
dc.subjectBiomarkers
dc.subjectCyclic voltammetry
dc.subjectElectrochemical impedance spectroscopy
dc.subjectImmunosensors
dc.subjectNeurons
dc.subjectPlastic bottles
dc.subjectPlastic coatings
dc.subjectPolyethylene terephthalates
dc.subjectScanning electron microscopy
dc.subjectSpectroscopy
dc.subjectTin oxides
dc.subjectElectrode surfaces
dc.subjectImpedimetric biosensors
dc.subjectLabel-free immunosensor
dc.subjectLow detection limit
dc.subjectNeuron specific enolase
dc.subjectOptimal experimental conditions
dc.subjectPoly(glycidyl methacrylate)
dc.subjectStar polymers
dc.subjectElectrochemical electrodes
dc.titleAn ultrasensitive immunosensor based on tri-armed star poly(glycidyl methacrylate) polymer-coated ITO-PET electrode for detection of neuron-specific enolase in human serum
dc.typeArticle

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