Homofermantatif ve/veya heterofermantatif laktik asit bakterileri inokulantların Macar fiği-buğday karışımı silajların fermantasyon ve aerobik stabilite özellikleri üzerine etkileri
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Date
2012
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Namık Kemal Üniversitesi
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info:eu-repo/semantics/openAccess
Abstract
Bu çalışma homofermantatif ve/veya heterofermantatif laktik asit bakteri inokulantları ilavesinin, Macar Fiği-Buğday karışımı silajlarında fermantasyon, aerobik stabilite, hücre duvarı kapsamı ve in vitro organik madde sindirilebilirliği özellikleri üzerindeki etkilerinin saptanması amacı ile düzenlenmiştir. Araştırmada kullanılan Macar Fiği-Buğday karışımı hasılları süt olum:çiçeklenme başlangıcı döneminde hasat edilmiştir. Homofermantatif laktik asit bakterisi olarak inokulant 1188 (Pioneer® , USA) ve heterofermantatif laktik asit bakterisi olarak inokulant 11A44 (Pioneer® , USA) kullanılmıştır. İnokulantlar silajlara 6,00 log10 koloni form ünite/g düzeyinde katılmışlardır. Macar Fiği-Buğday karışımları yalnızca gaz çıkışına olanak tanıyan, 1,0 litrelik özel kavanozlara silolanmıştır. Kavanozlar laboratuvar koşullarında 25±2°C' de depolanmışlardır. Silolamadan sonraki 2, 4, 8 ve 45. günlerde her gruptan 4'er kavanoz açılarak silajlarda kimyasal ve mikrobiyolojik analizler yapılmıştır. Silolama döneminin sonunda açılan tüm silajlara 5 gün süre ile aerobik stabilite testi uygulanmıştır. Ayrıca bu silajların, in vitro organik madde sindirilebilirliği saptanmıştır. Sonuç olarak homofermantatif laktik asit bakteri inokulantı Macar Fiği - Buğday silajlarının fermantasyon özelliklerini arttırmış ancak aerobik stabilitelerini düşürmüştür (P<0.05). Bununla birlikte heterofermantatif laktik asit bakteri inokulantı ile muamale edilmiş Macar Fiği-Buğday silajlarının asetik asit içeriği (P<0.05) ile aerobik stabilitesi (P<0.01) artmıştır. İn vitro organik madde sindirilebilirliği üzerine muamelelerin etkisi önemsiz (P>0.05) bulunmuştur.
This study was carried out to determine the effects of homofermentative (LAB) inoculants and/or heterofermentative lactic acid bacteria on the fermentation, aerobic stability and in vitro organic matter digestability characteristics of Hungarian vetch-Wheat mixture silages. Hungarian vetch-Wheat mixtures were harvested at early bloom: milking stage. Inoculant 1188 (Pioneer® , USA), was used as homofermentative lactic acid bacteria and inoculant 11A44 (Pioneer® , USA) was used as heterofermentative lactic acid bacteria inoculant. Inoculants were applied to silages at 6.00 log10 cfu/g levels. After treatment, the chopped Hungarian vetch - Wheat was ensiled in 1.0 liter special anaerobic jars, equipped with a lid enabling gas release only. The jars were stored at 25±2°C under laboratory conditions. Three jars from each group were sampled for chemical and microbiological analysis 2, 4, 8 and 45 days after ensiling. At the end of the ensiling period all silages were subjected to an aerobic stability test for 5 days. In addition, in vitro organic matter digestability of these silages were determined. Homofermentative lactic acid bacteria inoculants increased characteristics of fermentation but impaired aerobic stability of Hungarian vetch-Wheat silages (P<0.05). However, application of heterofermentative lactic acid bacteria increased the concentration of acetic acid (P<0.05) and aerobic stability (P<0.01) of Hungarian vetch-Wheat silages. There was no (P>0.05) treatment effect on any variables measured on in vitro organic matter digestability.
This study was carried out to determine the effects of homofermentative (LAB) inoculants and/or heterofermentative lactic acid bacteria on the fermentation, aerobic stability and in vitro organic matter digestability characteristics of Hungarian vetch-Wheat mixture silages. Hungarian vetch-Wheat mixtures were harvested at early bloom: milking stage. Inoculant 1188 (Pioneer® , USA), was used as homofermentative lactic acid bacteria and inoculant 11A44 (Pioneer® , USA) was used as heterofermentative lactic acid bacteria inoculant. Inoculants were applied to silages at 6.00 log10 cfu/g levels. After treatment, the chopped Hungarian vetch - Wheat was ensiled in 1.0 liter special anaerobic jars, equipped with a lid enabling gas release only. The jars were stored at 25±2°C under laboratory conditions. Three jars from each group were sampled for chemical and microbiological analysis 2, 4, 8 and 45 days after ensiling. At the end of the ensiling period all silages were subjected to an aerobic stability test for 5 days. In addition, in vitro organic matter digestability of these silages were determined. Homofermentative lactic acid bacteria inoculants increased characteristics of fermentation but impaired aerobic stability of Hungarian vetch-Wheat silages (P<0.05). However, application of heterofermentative lactic acid bacteria increased the concentration of acetic acid (P<0.05) and aerobic stability (P<0.01) of Hungarian vetch-Wheat silages. There was no (P>0.05) treatment effect on any variables measured on in vitro organic matter digestability.
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Keywords
Macar Fiği - Buğday karışımları, Laktik asit bakteri inokulantları, Fermantasyon, Aerobik stabilite, Hücre duvarı kapsamı, in vitro organik madde sindirilebilirliği, Hungarian vetch - Wheat mixtures, Lactic acid bacterial inoculants, Enzyme, Fermentation, Aerobic stability, Cell wall content, in vitro organic matter digestability
