Investigation of the relationship between the Th17/IL-23 pathway and innate-adaptive immune system in TNBS-induced colitis in rats

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dc.authorid0000-0002-2500-7781
dc.authorscopusid55838809000
dc.authorscopusid57211127362
dc.authorscopusid22985194700
dc.authorwosidkaraboga, ihsan/AAZ-9840-2020
dc.authorwosidKARACA, Turan/ABD-6669-2020
dc.contributor.authorKaraboğa, İhsan
dc.contributor.authorDemirtaş, Selim
dc.contributor.authorKaraca, Turan
dc.date.accessioned2022-05-11T14:48:35Z
dc.date.available2022-05-11T14:48:35Z
dc.date.issued2017
dc.departmentYüksekokullar, Sağlık Yüksekokulu, Acil Yardım ve Afet Yönetimi Bölümü
dc.description.abstractObjective(s): This study was aimed at investigating immune activations of the 2,4,6-trinitrobenzene sulfonic acid(TNBS)-induced colitis model in colonic mucosa by immunohistochemical and Western blot methods. Materials and Methods: For this purpose, 16 female Wistar albino rats were divided into two random groups of control (n=8) and colitis (n=8). The experimental colitis model was induced by intracolonicadministration of TNBS (25 mg/rat). Control animals received only rectal saline for the same time. The animals were sacrificed on the 15th day after TNBS administration, and colon tissue was removed and examined morphologically. Colon samples were stained immunohistochemically with anti-CD3, anti-CD4, anti-CD5, anti-CD8, anti-CD11b, anti-CD45, anti-TNF-alpha, anti-IL-17, anti-IL-22 and anti-IL-23 antibodies. Additionally, the colonic tissue IL-17 and IL-22 expressions were examined by the Western blot method. Results: In the experimental results, it was determined that there was a significant decrease in body weight and an increase in colon weight in the colitis group when comparing initial experiments. The colon tissue ulcerations, inflammation, crypt loss and Goblet cell loss were observed in the colitis group in microscopic examinations. The immunohistochemical positive cell numbers significantly increased in the colitis group. The immunoreactive lymphocytes in the propria, intracryptal and submucosal layers were found to be increased in the colitis group of rats. In addition, IL-17 and IL-23 expressions were increased in colitis colon mucosa found by Western blot analysis. Conclusion: The Th17/IL-23 pathway and IL-22 serve important roles in the pathogenesis of ulcerative colitis, and will be further examined by study.
dc.description.sponsorshipResearch Fund of the Trakya UniversityTrakya University [2014/20]
dc.description.sponsorshipThe results presented in this paper were part of Doctoral thesis. The project was supported by Research Fund of the Trakya University. Project number: 2014/20 funding agency.
dc.identifier.doi10.22038/IJBMS.2017.9108
dc.identifier.endpage879
dc.identifier.issn2008-3866
dc.identifier.issn2008-3874
dc.identifier.issue8en_US
dc.identifier.pmid29085578
dc.identifier.scopus2-s2.0-85026908536
dc.identifier.scopusqualityQ2
dc.identifier.startpage870
dc.identifier.urihttps://doi.org/10.22038/IJBMS.2017.9108
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10660
dc.identifier.volume20
dc.identifier.wosWOS:000409211300005
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKaraboğa, İhsan
dc.language.isoen
dc.publisherMashhad Univ Med Sciences
dc.relation.ispartofIranian Journal of Basic Medical Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectImmunohistochemistry
dc.subjectRat
dc.subject2,4,6-trinitrobenzene
dc.subjectsulfonic acid
dc.subjectUlcerative colitis
dc.subjectWestern blot
dc.subjectCd8(+) T-Cells
dc.subjectUlcerative-Colitis
dc.subjectAdhesion Molecules
dc.subjectTh17 Cells
dc.subjectTnf-Alpha
dc.subjectExpression
dc.subjectCytokine
dc.subjectIl-22
dc.subjectInflammation
dc.subjectEffector
dc.titleInvestigation of the relationship between the Th17/IL-23 pathway and innate-adaptive immune system in TNBS-induced colitis in rats
dc.typeArticle

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