Prenatal immobility stress: Relationship with oxidative stress, inflammation, apoptosis, and intrauterine growth restriction in rats

Basit Öğe Kaydı
dc.authoridOkuyan, Hamza Malik/0000-0001-7616-3330
dc.contributor.authorKaya, Sinem Albayrak
dc.contributor.authorOkuyan, Hamza Malik
dc.contributor.authorErboga, Zeynep Fidanol
dc.contributor.authorGuzel, Savas
dc.contributor.authorYilmaz, Ahsen
dc.contributor.authorKaraboga, Ihsan
dc.date.accessioned2024-10-29T17:58:16Z
dc.date.available2024-10-29T17:58:16Z
dc.date.issued2023
dc.departmentTekirdağ Namık Kemal Üniversitesien_US
dc.description.abstractBackgroundPrenatal stress is a significant risk factor affecting pregnant women and fetal health. In the present study, we aimed to investigate the effect of immobility stress at different periods of pregnancy on oxidative stress, inflammation, placental apoptosis and intrauterine growth retardation in rats.MethodsFifty adult virgin female Wistar albino rats were used. Pregnant rats were exposed to 6 h/day immobilization stress in a wire cage at different stages of pregnancy. Groups I and II (Day 1-10 stress group) were sacrificed on the 10th day of pregnancy, and Group III, Group IV (10-19th-day stress group), and Group V (1-19th-day stress group) were sacrificed on the 19th day of pregnancy. Inflammatory cytokines, including interleukin-6 (IL-6) and interleukin-10 (IL-10), serum corticotropin-releasing hormone (CRH), and corticosterone levels were measured by enzyme-linked immunosorbent assay. Malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) levels in the placenta were spectrophotometrically measured. Histopathological analyses of the placenta were evaluated by hematoxylin and eosin staining. Tumor necrosis factor-alpha (TNF-a) and caspase-3 immunoreactivity in placenta tissues were determined by the indirect immunohistochemical method. Placental apoptosis was determined by the TUNEL staining method.ResultsWe found that the immobility stress during pregnancy significantly increased serum corticosterone levels. Our results showed that the immobility stress diminished the number and weight of fetuses in rats compared to the non-stress group. The immobility stress caused significant histopathological changes in the connection zone and labyrinth zone and increased placental TNF-a and caspase-3 immunoreactivity and placental apoptosis. In addition, immobility stress significantly increased the levels of pro-inflammatory IL-6 and MDA and caused a significant decrease in the levels of antioxidant enzymes such as SOD, CAT, and anti-inflammatory IL-10.ConclusionsOur data suggest that immobility stress causes intrauterine growth retardation by activating the hypothalamic-pituitary-adrenal axis and deteriorating placental histomorphology and deregulating inflammatory and oxidative processes.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkiye (TUBITAK) [118S624]en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkiye (TUBITAK),Grant/Award Number: 118S624en_US
dc.identifier.doi10.1002/bdr2.2205
dc.identifier.endpage1410en_US
dc.identifier.issn2472-1727
dc.identifier.issue15en_US
dc.identifier.pmid37403489en_US
dc.identifier.scopus2-s2.0-85164346292en_US
dc.identifier.startpage1398en_US
dc.identifier.urihttps://doi.org/10.1002/bdr2.2205
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14173
dc.identifier.volume115en_US
dc.identifier.wosWOS:001024196700001en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofBirth Defects Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectimmobility stressen_US
dc.subjectinflammationen_US
dc.subjectintrauterine growth retardationen_US
dc.subjectoxidative stressen_US
dc.subjectplacental apoptosisen_US
dc.titlePrenatal immobility stress: Relationship with oxidative stress, inflammation, apoptosis, and intrauterine growth restriction in ratsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu