Carboxyethylsilanetriol-Coated Magnetic Nanoparticles as an Ultrasensitive Immunoplatform for Electrochemical Magnetosensing of Cotinine

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dc.authoridAYDIN, MUHAMMET/0000-0002-8716-9970
dc.contributor.authorAydin, Muhammet
dc.contributor.authorAydin, Elif Burcu
dc.contributor.authorSezginturk, Mustafa Kemal
dc.date.accessioned2024-10-29T17:58:31Z
dc.date.available2024-10-29T17:58:31Z
dc.date.issued2024
dc.departmentTekirdağ Namık Kemal Üniversitesien_US
dc.description.abstractIn the present study, an innovative and simple electrochemical magneto biosensor based on carboxyethylsilanetriol-modified iron oxide (Fe3O4) magnetic nanoparticles was designed for ultrasensitive and specific analysis of cotinine, an important marker of smoking. Anticotinine antibodies were covalently immobilized on carboxylic acid-modified magnetic nanoparticles, and the cotinine-specific magnetic nanoparticles created a specific surface on the working electrode surface. The use of magnetic nanoparticles as an immobilization platform for antibodies provided a large surface area for antibody attachment and increased sensitivity. In addition, the advantages of the new immobilization platform were reusing the working electrode numerous times, recording repeatable and reproducible signals, and reducing the necessary volume of biomolecules. The specific interaction between cotinine and cotinine-specific antibody-attached magnetic nanoparticles restricted the electron transfer of the redox probe and changed the impedimetric response of the electrode correlated to the concentration of cotinine. The magneto biosensor had a wide detection range (2-300 pg/mL), a low LOD (606 fg/mL), and an acceptable recovery (97.24-105.31%) in real samples. In addition, the current biosensor's measurement results were in good agreement with those found by the standard liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA) methods. These results showed that a simple impedimetric immunosensing platform was generated for the cotinine analysis.en_US
dc.description.sponsorshipTekirdag Namik Kemal ?niversitesi [NKUBAP.00, 23.466]; Scientific and Technological Research Council of Tekirdagy Namimath;k Kemal Universityen_US
dc.description.sponsorshipThe authors are very grateful for the financial support from the Scientific and Technological Research Council of Tekirdagy Nam & imath;k Kemal University (NKUBAP.00.GA.23.466).en_US
dc.identifier.doi10.1021/acsbiomaterials.3c01872
dc.identifier.endpage2580en_US
dc.identifier.issn2373-9878
dc.identifier.issue4en_US
dc.identifier.pmid38529538en_US
dc.identifier.scopus2-s2.0-85188697899en_US
dc.identifier.startpage2567en_US
dc.identifier.urihttps://doi.org/10.1021/acsbiomaterials.3c01872
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14367
dc.identifier.volume10en_US
dc.identifier.wosWOS:001191249600001en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Chemical Socen_US
dc.relation.ispartofAcs Biomaterials Science & Engineeringen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcotinineen_US
dc.subjecttobacco smokingen_US
dc.subjectelectrochemicalsensingen_US
dc.subjectcarboxyethylsilanetriolen_US
dc.titleCarboxyethylsilanetriol-Coated Magnetic Nanoparticles as an Ultrasensitive Immunoplatform for Electrochemical Magnetosensing of Cotinineen_US
dc.typeArticleen_US

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