Bioinformatic Analysis and in Vitro Validation of Let-7b and Let-7c in Breast Cancer

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dc.authorscopusid56497061100
dc.contributor.authorBozgeyik, Esra
dc.date.accessioned2022-05-11T14:05:11Z
dc.date.available2022-05-11T14:05:11Z
dc.date.issued2020
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalı
dc.description.abstractMembers of the let-7 family of miRNAs are well-known with their tumor suppressor properties as they are expressed at low levels in several types of human malignancies. Among them, let-7b and let-7c have gained special attention due their broad significance. Although the role of let-7b and let-7c have been widely reported in various types of cancers, their functional importance and role in oncogenic signaling of breast cancer is poorly investigated. Therefore, in the present study, prognostic and diagnostic significance of let-7b and let-7c in breast cancer and the effects these miRNAs on genes involved in cancer progression were determined by using several bioinformatics analysis and validated in vitro mimic assays, respectively. Using data of TCGA, OncomiR and dbDEMC 2.0, overall expression analysis of let-7b and let-7c was performed. The effect of let-7b and let-7c on genes involved in cancer progression was investigated by mimic transfection assays. We found that both let-7b and let-7c were significantly altered in breast cancer and associated with the clinicopathological findings of patients. Additionally, both let-7b and let-7c significantly altered oncogenic signaling in breast cancer cells. Consequently, both miRNAs might have fundamental roles in breast cancer progression and can be considered as potential targets for breast cancer therapy and diagnosis. © 2019 Elsevier Ltd
dc.identifier.doi10.1016/j.compbiolchem.2019.107191
dc.identifier.issn1476-9271
dc.identifier.pmid31901542
dc.identifier.scopus2-s2.0-85077141497
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.compbiolchem.2019.107191
dc.identifier.urihttps://hdl.handle.net/20.500.11776/4917
dc.identifier.volume84
dc.identifier.wosWOS:000510947400010
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorBozgeyik, Esra
dc.language.isoen
dc.publisherElsevier Ltd
dc.relation.ispartofComputational Biology and Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBreast cancer
dc.subjectLet-7
dc.subjectLet-7b
dc.subjectLet-7c
dc.subjectmiRNA
dc.subjectBioinformatics
dc.subjectCell signaling
dc.subjectDiagnosis
dc.subjectGenes
dc.subjectRNA
dc.subjectBioinformatic analysis
dc.subjectBioinformatics analysis
dc.subjectBreast Cancer
dc.subjectBreast cancer cells
dc.subjectExpression analysis
dc.subjectLet-7
dc.subjectmiRNA
dc.subjectTransfection assays
dc.subjectDiseases
dc.subjectmicroRNA
dc.subjectmirnlet7 microRNA, human
dc.subjecttumor marker
dc.subjectbiology
dc.subjectbreast tumor
dc.subjectfemale
dc.subjectgene expression profiling
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjecthuman
dc.subjectin vitro study
dc.subjecttumor cell line
dc.subjectBiomarkers, Tumor
dc.subjectBreast Neoplasms
dc.subjectCell Line, Tumor
dc.subjectComputational Biology
dc.subjectFemale
dc.subjectGene Expression Profiling
dc.subjectGene Expression Regulation, Neoplastic
dc.subjectHumans
dc.subjectIn Vitro Techniques
dc.subjectMicroRNAs
dc.titleBioinformatic Analysis and in Vitro Validation of Let-7b and Let-7c in Breast Cancer
dc.typeArticle

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