Fetal Tracheal Occlusion Increases Lung Basal Cells via Increased Yap Signaling

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dc.authorid0000-0001-9132-9271
dc.authorscopusid57323665200
dc.authorscopusid57323747400
dc.authorscopusid56601088000
dc.authorscopusid56985532100
dc.authorscopusid24167049200
dc.authorscopusid7004162740
dc.authorscopusid7005874031
dc.authorwosidOria, Marc/K-9599-2019
dc.contributor.authorSerapiglia, Vincent
dc.contributor.authorStephens, Chad A.
dc.contributor.authorJoshi, Rashika
dc.contributor.authorAydın, Emrah
dc.contributor.authorOria, Marc
dc.contributor.authorMarotta, Mario
dc.contributor.authorVarisco, Brian Michael
dc.date.accessioned2022-05-11T14:04:54Z
dc.date.available2022-05-11T14:04:54Z
dc.date.issued2022
dc.departmentFakülteler, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Çocuk Cerrahisi Ana Bilim Dalı
dc.description.abstractFetal endoscopic tracheal occlusion (FETO) is an emerging surgical therapy for congenital diaphragmatic hernia (CDH). Ovine and rabbit data suggested altered lung epithelial cell populations after tracheal occlusion (TO) with transcriptomic signatures implicating basal cells. To test this hypothesis, we deconvolved mRNA sequencing (mRNA-seq) data and used quantitative image analysis in fetal rabbit lung TO, which had increased basal cells and reduced ciliated cells after TO. In a fetal mouse TO model, flow cytometry showed increased basal cells, and immunohistochemistry demonstrated basal cell extension to subpleural airways. Nuclear Yap, a known regulator of basal cell fate, was increased in TO lung, and Yap ablation on the lung epithelium abrogated TO-mediated basal cell expansion. mRNA-seq of TO lung showed increased activity of downstream Yap genes. Human lung specimens with congenital and fetal tracheal occlusion had clusters of subpleural basal cells that were not present in the control. TO increases lung epithelial cell nuclear Yap, leading to basal cell expansion.
dc.identifier.doi10.3389/fped.2021.780166
dc.identifier.issn2296-2360
dc.identifier.pmid35280447
dc.identifier.scopus2-s2.0-85126259794
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.3389/fped.2021.780166
dc.identifier.urihttps://hdl.handle.net/20.500.11776/4829
dc.identifier.volume9
dc.identifier.wosWOS:000769802800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Emrah
dc.language.isoen
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers In Pediatrics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectfetal tracheal occlusion
dc.subjectcongenital diaphragm hernia
dc.subjectlung development
dc.subjectbasal cell
dc.subjectYap (Hippo) signaling
dc.subjectmechanotransduction
dc.subjectCongenital Diaphragmatic-Hernia
dc.subjectRabbit Model
dc.subjectExpression
dc.subjectDifferentiation
dc.subjectAirway
dc.subjectUtero
dc.subjectMice
dc.titleFetal Tracheal Occlusion Increases Lung Basal Cells via Increased Yap Signaling
dc.typeArticle

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