Gas6 expression and tyrosine kinase axl sky receptors: Their relation with tumor stage and grade in patients with bladder cancer

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dc.authorscopusid36151868000
dc.authorscopusid55955553900
dc.authorscopusid56566747500
dc.authorscopusid56707153300
dc.authorscopusid15053902900
dc.authorscopusid6602772540
dc.authorscopusid10045459300
dc.contributor.authorAkgül, Murat
dc.contributor.authorBaykan, Özgür
dc.contributor.authorÇa?man, Zeynep
dc.contributor.authorÖzyürek, Mustafa
dc.contributor.authorTinay, İlker
dc.contributor.authorAkbal, Cem
dc.contributor.authorTürkeri, Levent
dc.date.accessioned2022-05-11T14:04:57Z
dc.date.available2022-05-11T14:04:57Z
dc.date.issued2021
dc.departmentFakülteler, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Üroloji Ana Bilim Dalı
dc.description.abstractObjectives: It has been shown that the dysregulation of tyrosine kinase Axl receptor and its ligand growth arrest-specific gene (Gas6) are associated with poor prognosis in various types of tumors but there is not enough study about their importance in bladder cancer (BC). We evaluated the relation of Gas6 gene expression and tyrosine- kinase Axl and Sky (Tyro 3) receptors with tumor stage and grade in patients with BC. Material and Methods: The study group consists of 55 patients whose transurethral resection of bladder (TUR-B) has been performed due to BC and the control group consists of 12 patients with normal bladder mucosa. In tissues mRNAs of Gas6, Axl, and Sky receptors were examined by quantitative (Real-Time) PCR (qPCR). Protein expression was measured by immunohistochemistry. Plasma Gas6 protein levels were compared with control group by ELISA method. Results: Patients with BC were grouped as Ta low (n=17), Ta high (n=5), T1 low (n=9), T1 high (n=8) and T2 (n=16) according to their TUR-B pathologies. The qPCR analysis showed that the expression of Gas6 gene and Axl receptor is higher in the tumor-positive group and the immune-histochemical showed that the bladder samples of the tumor-positive group stained significantly positive. When the patients are grouped according to the TUR-B pathologies, a statistical significant difference was observed among groups in the qPCR analysis ratios of Gas6 gene and Axl receptor by (p < 0.05) but no significance was found for Sky receptor (p > 0.05). When Gas6 protein levels in plasma samples were compared by ELISA method, a statistical significance was determined among groups (p = 0.001). Conclusions: Our findings indicate that mRNAs of Gas6 and Axl receptor are closely related to tumor stage and grade in patients with BC. Further studies are needed for understanding the role of Gas6 and its receptors on the neoplastic transformation in terms of novel biomarkers and potential therapeutic targets. © 2021 Edizioni Scripta Manent s.n.c.. All rights reserved.
dc.description.sponsorshipSAG-C-TUP-130612-0208
dc.description.sponsorshipThis study was supported by Marmara University Scientific Research Projects Committee (Grant number: SAG-C-TUP-130612-0208).
dc.identifier.doi10.4081/aiua.2021.2.148
dc.identifier.endpage152
dc.identifier.issn1124-3562
dc.identifier.issue2en_US
dc.identifier.pmid34286546
dc.identifier.scopus2-s2.0-85111787602
dc.identifier.scopusqualityQ3
dc.identifier.startpage148
dc.identifier.urihttps://doi.org/10.4081/aiua.2021.2.148
dc.identifier.urihttps://hdl.handle.net/20.500.11776/4848
dc.identifier.volume93
dc.identifier.wosWOS:000704496200005
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAkgül, Murat
dc.language.isoen
dc.publisherPage Press Publications
dc.relation.ispartofArchivio Italiano di Urologia e Andrologia
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAxl
dc.subjectBladder cancer
dc.subjectGas6
dc.subjectSky
dc.subjectTyro3
dc.subjectAxl protein
dc.subjectgrowth arrest specific protein 6
dc.subjectmessenger RNA
dc.subjectprotein tyrosine kinase
dc.subjectSky protein
dc.subjectunclassified drug
dc.subjectoncoprotein
dc.subjectprotein tyrosine kinase
dc.subjectsignal peptide
dc.subjectadult
dc.subjectArticle
dc.subjectbladder cancer
dc.subjectblood sampling
dc.subjectcancer grading
dc.subjectcancer patient
dc.subjectcancer staging
dc.subjectcontrolled study
dc.subjectenzyme linked immunosorbent assay
dc.subjectfemale
dc.subjectgene expression
dc.subjecthuman
dc.subjectimmunohistochemistry
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectmiddle aged
dc.subjectprotein expression
dc.subjectreal time polymerase chain reaction
dc.subjecttransurethral resection
dc.subjectbladder tumor
dc.subjectgenetics
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectIntercellular Signaling Peptides and Proteins
dc.subjectProto-Oncogene Proteins
dc.subjectReceptor Protein-Tyrosine Kinases
dc.subjectUrinary Bladder Neoplasms
dc.titleGas6 expression and tyrosine kinase axl sky receptors: Their relation with tumor stage and grade in patients with bladder cancer
dc.typeArticle

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