Effect of ß-glucan from Euglena gracilis as an antioxidant on goat semen cryopreservation

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dc.contributor.authorUstuner, B.
dc.contributor.authorYilmaz, M. M.
dc.contributor.authorHuraydin, O.
dc.contributor.authorCimen, T.
dc.contributor.authorSonat, F. Ak
dc.contributor.authorAktar, A.
dc.contributor.authorGokce, E.
dc.date.accessioned2024-10-29T17:58:51Z
dc.date.available2024-10-29T17:58:51Z
dc.date.issued2024
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractThis study aimed to determine the influence of different doses of beta-glucan on post-thaw spermatological parameters, lipid peroxidation, and total antioxidant activity of buck semen. In the non-breeding season, semen was collected from bucks twice weekly. After then, ejaculates were pooled and divided into four equal aliquots: beta-glucan concentrations of 1 mM (beta G1), 2 mM (beta G2), and 4 mM (beta G4), and a control group without antioxidants. Each sample group was diluted for cryopreservation using a dilution method involving two steps. The experimental groups were then evaluated for several parameters including sperm motility, plasma membrane functional integrity [hypoosmotic swelling test (HOST)], damaged acrosome rate [FITC-Pisum sativum agglutinin (FITC-PSA)], DNA integrity [terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)], mitochondrial membrane potential using JC-1, evaluation of lipid peroxidation, and determination of total antioxidant activity. The post-thaw motility and plasma functional integrity of the control group were significantly lower than those values in the beta G groups (P < 0.05). Although the numerically greatest acrosome damage was detected in the control group, it was only statistically different from beta G1 and beta G4 (P<0.05). While the DNA fragmentation rate of the control group was higher than beta G4 group (P<0.05), it was similar to beta G1 and beta G2 groups (P>0.05). There was no statistical difference among all the groups regarding low mitochondrial membrane potential, MDA, and TAC rates. In line with our results, supplementation of 1mM, 2 mM and 4 mM beta-glucan to freezing extender improves the post-thaw spermatological characteristics of goat semen.
dc.identifier.doi10.12681/jhvms.34504
dc.identifier.issn1792-2720
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85200322241
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.12681/jhvms.34504
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14529
dc.identifier.volume75
dc.identifier.wosWOS:001282354400016
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherHellenic Veterinary Medical Soc
dc.relation.ispartofJournal of The Hellenic Veterinary Medical Society
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGoat
dc.subjectss-glucan
dc.subjectmitochondrial membrane potential
dc.subjectDNA fragmentation
dc.subjectantioxidant activity
dc.titleEffect of ß-glucan from Euglena gracilis as an antioxidant on goat semen cryopreservation
dc.typeArticle

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