Ion chromatographic method for the determination of orotic acid in urine

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dc.authorid0000-0002-8550-0105
dc.authorid0000-0003-2477-0694
dc.authorscopusid57189851629
dc.authorscopusid57189470687
dc.authorscopusid8283704400
dc.authorscopusid55623526000
dc.authorwosidZeydanli, Damla/ABB-8507-2020
dc.authorwosidDestanoğlu, Orhan/ABB-4428-2020
dc.authorwosidCansever, Mehmet Serif/AAU-8758-2020
dc.contributor.authorDestanoglu, Orhan
dc.contributor.authorZeydanli, Damla
dc.contributor.authorCansever, Mehmet Şerif
dc.contributor.authorYılmaz, Gülcin Gumus
dc.date.accessioned2022-05-11T14:47:18Z
dc.date.available2022-05-11T14:47:18Z
dc.date.issued2018
dc.departmentMeslek Yüksekokulları, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümü
dc.description.abstractExcess urinary orotic acid excretion occurs in patients with some inborn errors of metabolic pathways such as pyrimidine synthesis and urea cycle. Thus, rapid diagnosis of orotic aciduria has a vital importance for patients. In this paper, a novel method for determination of orotic acid in urine samples by ion chromatography with suppressed conductivity detection was investigated. The separation of orotic acid from urine matrix was accomplished by using an anion exchange column with optimized isocratic eluent program which utilized 50 mM NaOH. The other chromatographic conditions were as follows: the suppressor current was 31 mA; the flow rate of mobile phase was 0.25 mL min(-1); the column temperature was 30 degrees C; sample loop volume was 10 mu L. Under optimized conditions, the limit of detection was 0.2 mu mol L-1. Dramatically elevated orotic acid concentration was observed on pathological urine samples comparing to healthy urines, as expected. There are a good many advantages of the proposed method, but using an environmentally friendly reagent free system, no organic solvent employment and its quick nature, and being a sensitive and reliable method are the most obvious ones. The proposed method, therefore, may be utilized as an alternative technique for clinical laboratories.
dc.description.sponsorshipUnit for Scientific Research Project of Istanbul Technical University
dc.description.sponsorshipThe authors would like to thank the Unit for Scientific Research Project of Istanbul Technical University for providing financial support.
dc.identifier.doi10.1016/j.ab.2018.09.016
dc.identifier.endpage14
dc.identifier.issn0003-2697
dc.identifier.issn1096-0309
dc.identifier.pmid30267708
dc.identifier.scopus2-s2.0-85054076301
dc.identifier.scopusqualityQ3
dc.identifier.startpage9
dc.identifier.urihttps://doi.org/10.1016/j.ab.2018.09.016
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10473
dc.identifier.volume563
dc.identifier.wosWOS:000450541700002
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorCansever, Mehmet Şerif
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Science
dc.relation.ispartofAnalytical Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectOrotic acid
dc.subjectIon chromatography
dc.subjectMetabolic disorder
dc.subjectUrea cycle defect
dc.subjectArgininosuccinic aciduria
dc.subjectUrine
dc.subjectRapid-Determination
dc.subjectDiagnostic-Value
dc.subjectInborn-Errors
dc.subjectSeparation
dc.subjectDisorders
dc.titleIon chromatographic method for the determination of orotic acid in urine
dc.typeArticle

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