An impedimetric immunosensor for highly sensitive detection of IL-8 in human serum and saliva samples: A new surface modification method by 6-phosphonohexanoic acid for biosensing applications

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dc.authorscopusid57194273220
dc.authorscopusid6506764918
dc.authorwosidAYDIN, Elif Burcu/ABA-9053-2020
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:48:13Z
dc.date.available2022-05-11T14:48:13Z
dc.date.issued2018
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractIn this study, we fabricated a sensitive and label-free impedimetric immunosensor based on 6-phosphonohexanoic acid (PHA) modified ITO electrode for detection of interleukin-8 (IL-8) in human serum and saliva. PHA was first employed to cancer biomarker sensing platform. Anti-IL-8 antibody was used as a biorecognition element and the detection principle of this immunosensor was based on monitoring specific interaction between anti-IL-8 antibody and IL-8 antigen. The morphological characterization of each electrode modification step was analyzed by scanning electron microscopy (SEM), SEM-energy dispersive X-ray spectroscopy (EDX) and atomic force microscopy (AFM) while electrochemical characterization was performed by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and single frequency impedance (SFI) techniques. Moreover, the antibody immobilization on the electrode surface was proved Fourier-transform infrared spectroscopy (FTIR) and Raman Spectroscopy. This proposed impedimetric immunosensor exhibited good performances with a wide linear in the range from 0.02 pg/mL to 3 pg/mL as well as a relative low detection limit of 6 fg/mL. The impedimetric immunosensor had a good specificity, stability and reproducibility. This study proved that PHA was a suitable interface material to fabricate an electrochemical biosensor.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113 Z 678]
dc.description.sponsorshipThe financial support from TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 113 Z 678) is gratefully acknowledged.
dc.identifier.doi10.1016/j.ab.2018.05.030
dc.identifier.endpage52
dc.identifier.issn0003-2697
dc.identifier.issn1096-0309
dc.identifier.pmid29902421
dc.identifier.scopus2-s2.0-85048268062
dc.identifier.scopusqualityQ3
dc.identifier.startpage44
dc.identifier.urihttps://doi.org/10.1016/j.ab.2018.05.030
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10614
dc.identifier.volume554
dc.identifier.wosWOS:000439741300007
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Elif Burcu
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Science
dc.relation.ispartofAnalytical Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectInterleukin-8
dc.subject6-Phosphonohexanoic acid
dc.subjectIndium tin oxide
dc.subjectElectrochemical impedance spectroscopy
dc.subjectSingle frequency impedance spectroscopy
dc.subjectIto-Based Immunosensor
dc.subjectLabel-Free
dc.subjectElectrochemical Immunosensor
dc.subjectCancer Biomarker
dc.subjectInterleukin-8
dc.subjectPolymer
dc.subjectImmobilization
dc.subjectDiagnostics
dc.subjectElectrodes
dc.subjectSensor
dc.titleAn impedimetric immunosensor for highly sensitive detection of IL-8 in human serum and saliva samples: A new surface modification method by 6-phosphonohexanoic acid for biosensing applications
dc.typeArticle

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