Novel thiosemicarbazone derivative 17B interferes with the cell cycle progression and induce apoptosis through modulating downstream signaling pathways

Basit Öğe Kaydı
dc.authorid0000-0002-4667-4655
dc.authorid0000-0002-8726-3182
dc.authorid0000-0003-1483-2580
dc.authorscopusid56497061100
dc.authorscopusid57212340481
dc.authorscopusid56190917500
dc.authorscopusid36772919600
dc.authorscopusid52363775100
dc.authorscopusid6701544588
dc.authorwosidArman, Kaifee/A-5977-2015
dc.authorwosidBozgeyik, Esra/AAE-2062-2022
dc.authorwosidİyidoğan, Ayşegül/AAK-6549-2021
dc.authorwosidbozgeyik, ibrahim/AAI-2723-2020
dc.contributor.authorBozgeyik, Esra
dc.contributor.authorTaşdemir Kahraman, Demet
dc.contributor.authorArman, Kaifee
dc.contributor.authorBozgeyik, İbrahim
dc.contributor.authorKaraküçük İyidoğan, Ayşegül
dc.contributor.authorÇakmak, Ecir Ali
dc.date.accessioned2022-05-11T14:10:05Z
dc.date.available2022-05-11T14:10:05Z
dc.date.issued2020
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalı
dc.departmentFakülteler, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Genetik Ana Bilim Dalı
dc.departmentFakülteler, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, İç Hastalıkları Ana Bilim Dalı
dc.description.abstractThiosemicarbazones (TSCs) are interesting group of chemical compounds that received significant levels of attention due their wide range of pharmacological effects including antibacterial, antiviral, and especially anti-tumor activities. Several thiosemicarbazone derivatives have been extensively reported recently with their anti-tumor properties but designing and developing novel thiosemicarbazone derivatives with more potent chemotherapeutic activities is of great interest for cancer future cancer therapy. Thus, here we aimed to demonstrate as yet undetermined anti-cancer properties of novel thiosemicarbazone derivative 17B. Viability of cells was determined using MTT assay and LDH activities were analyzed using lactate dehydrogenase activity assay. Apoptosis were assayed using Annexin V-FITC and PI double staining method and cell cycle analysis was achieved by using PI staining with fluorescence-activated cell sorting and migration capacities of cells were determined by wound healing assay. As a result, 17B limited cell viability and showed cytotoxic effects in a dose-dependent manner in A549, MCF7 and U2OS cells. In addition, it inhibited progression through cell cycle by interfering with the Gl/S transition and triggered apoptosis by modulating expression levels of pro-apoptotic and anti-apoptotic mediators in MCF7 and U2OS cells. Also, 17B significantly impaired the migration of cancer cells and delayed wound healing in all cells. Consequently, findings of the present study have strongly indicated that 17B might be a novel anti-cancer agent for the treatment of breast cancer and osteosarcoma but not for lung cancer. Our results have provided mechanistic insights into anti-cancer properties of a novel thiosemicarbazone derivative 17B.
dc.identifier.doi10.1016/j.genrep.2019.100578
dc.identifier.issn2452-0144
dc.identifier.scopus2-s2.0-85076476401
dc.identifier.scopusqualityQ4
dc.identifier.urihttps://doi.org/10.1016/j.genrep.2019.100578
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5277
dc.identifier.volume18
dc.identifier.wosWOS:000513174200029
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorBozgeyik, Esra
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofGene Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAnti-cancer agent
dc.subjectCancer
dc.subjectCytotoxicity
dc.subjectThiosemicarbazone derivative
dc.subject17B
dc.subjectAntitumor-Activity
dc.subjectMigration
dc.subjectProliferation
dc.titleNovel thiosemicarbazone derivative 17B interferes with the cell cycle progression and induce apoptosis through modulating downstream signaling pathways
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
5277-2.pdf
Boyut:
122.58 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Tıp Fakültesi Koleksiyonu