Molecular Characterisation of 28S Region of Xiphinema index (Dorylaimida: Longidoridae) from Türkiye

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dc.contributor.authorÖztürk, Lerzan
dc.contributor.authorElekcioğlu, İbrahim Halil
dc.date.accessioned2024-10-29T17:55:16Z
dc.date.available2024-10-29T17:55:16Z
dc.date.issued2024
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractXiphinema index (Dorylaimida: Longidoridae) is a virus vector nematode and is highly distributed worldwide. In this study, the DNA of the Xiphinema index nematode was subject to Polymerase chain reaction (PCR) using D2A and D3B primers to analyse the 28S region. The resulting sequence data were then subjected to a BLAST check in order to ascertain the similarity to other species. The X. index population in the study was recovered from a fig (Ficus carica L.) plantation in Tekirdağ, Türkiye. The DNA of specimens was amplified with D2A and D3B primers to perform phylogenetic analysis. At first, the nematodes were identified morphologically and then molecularly. D2A and D3B primers were used in the study. After PCR, amplified DNA was sequenced for molecular purposes, and data from the D2-D3 region of the 28S rRNA molecule of X. index were subjected to GenBank sequence comparison using BLAST (Basic Local Alignment Search Tool). The local sequence submitted to GenBank with accession number PQ165044 showed identity (98.26-99.81%) with several Xiphinema index hits. Local X. index sequence clustered close with Spanish (HM921406, HM921400, HM921399, HM921398, HM921363, HM921349, HM921347 and HM921364) and Crete/Greece (KJ802882) sequences in Neighbor-joining and Maximum likelihood analysis comparing the local sequence, 16 different X. index accessions and 13 accessions from various species.
dc.identifier.doi10.55507/gopzfd.1493529
dc.identifier.endpage123
dc.identifier.issn1300-2910
dc.identifier.issn2147-8848
dc.identifier.issue2en_US
dc.identifier.startpage117
dc.identifier.trdizinid1266577
dc.identifier.urihttps://doi.org/10.55507/gopzfd.1493529
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1266577
dc.identifier.urihttps://hdl.handle.net/20.500.11776/13924
dc.identifier.volume41
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofGaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTürkiye
dc.subjectFicus carica L.
dc.subject28S rRNA
dc.subjectDagger nematode
dc.titleMolecular Characterisation of 28S Region of Xiphinema index (Dorylaimida: Longidoridae) from Türkiye
dc.typeArticle

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