Fabrication of electrochemical immunosensor based on acid-substituted poly(pyrrole) polymer modified disposable ITO electrode for sensitive detection of CCR4 cancer biomarker in human serum

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dc.authorscopusid57194273220
dc.authorscopusid55571487900
dc.authorscopusid6506764918
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorAydın, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:07:25Z
dc.date.available2022-05-11T14:07:25Z
dc.date.issued2021
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractThis study described the first impedimetric immunosensor reported for the determination of CCR4, a new prostate cancer biomarker. This impedimetric immunosensor was constructed through the modification of disposable indium tin oxide (ITO) sheet with a conjugated pyrrole polymer P(Pyr-Pac) and subsequent immobilization of anti-CC chemokine receptor 4 (CCR4) antibodies. Acid-substituted poly(pyrrole) P(Pyr-Pac) polymer contained a lot of carboxyl groups on its end site, which were suitable for attachment of anti-CCR4 antibodies. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were chosen to investigate electrode preparation stages and, EIS was chosen to detect the CCR4 concentration. Anti-CCR4 antibody attached biosensing surface was highly selective to CCR4 antigen, the specific interaction resulted changes in electrochemical signal. Optimization studies containing polymer amount, anti-CCR4 antibody concentration, anti-CCR4 antibody immobilization time and anti-CCR4 antibody-CCR4 antigen interaction time were studied. The developed immunosensor displayed a linear increase with concentrations of CCR4 antigen (0.02–8 pg/mL) and a low detection limit of 6.4 fg/mL. In addition, this biosensor had great reproducibility and repeatability. Moreover, the designed biosensor was successfully used for the quantification of CCR4 antigen in serum samples. The recovery for the spiked serum samples was between 98.25% and 103.99%. The suggested immunosensor illustrated a good selectivity towards some interferents including different biomarkers. This study could establish a new approach for future cancer biomarker detection. © 2020 Elsevier B.V.
dc.description.sponsorshipÇanakkale Onsekiz Mart Üniversitesi, ÇOMÜ; Consejo Nacional para Investigaciones Científicas y Tecnológicas, CONICIT; FUK-2018-1396
dc.description.sponsorshipThis research was supported The Scientific and Technological Research Council of by ?anakkale Onsekiz Mart University, ?OM?-BAP (Grant number: FUK-2018-1396).
dc.description.sponsorshipThis research was supported The Scientific and Technological Research Council of by Çanakkale Onsekiz Mart University , ÇOMÜ-BAP (Grant number: FUK-2018-1396 ).
dc.identifier.doi10.1016/j.talanta.2020.121487
dc.identifier.issn0039-9140
dc.identifier.pmid33167207
dc.identifier.scopus2-s2.0-85089243137
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2020.121487
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5101
dc.identifier.volume222
dc.identifier.wosWOS:000589942600006
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Elif Burcu
dc.institutionauthorAydın, Muhammet
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofTalanta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAcid-substituted-polypyrrole P(Pyr-Pac) polymer
dc.subjectCC chemokine Receptor 4
dc.subjectConjugated polymers
dc.subjectAntibodies
dc.subjectAntigens
dc.subjectAromatic compounds
dc.subjectConjugated polymers
dc.subjectCyclic voltammetry
dc.subjectDiseases
dc.subjectElectrochemical electrodes
dc.subjectElectrochemical impedance spectroscopy
dc.subjectImmunosensors
dc.subjectTin oxides
dc.subjectAntibody concentration
dc.subjectAntibody immobilization
dc.subjectCancer biomarker detections
dc.subjectElectrochemical immunosensors
dc.subjectElectrochemical signals
dc.subjectElectrode preparation
dc.subjectImpedimetric immunosensors
dc.subjectSpecific interaction
dc.subjectBiomarkers
dc.subjectCCR4 protein, human
dc.subjectchemokine receptor CCR4
dc.subjectimmobilized antibody
dc.subjectindium tin oxide
dc.subjectpolymer
dc.subjectpyrrole derivative
dc.subjecttin derivative
dc.subjecttumor marker
dc.subjectelectrochemical analysis
dc.subjectelectrode
dc.subjectgenetic procedures
dc.subjecthuman
dc.subjectimmunoassay
dc.subjectlimit of detection
dc.subjectneoplasm
dc.subjectreproducibility
dc.subjectAntibodies, Immobilized
dc.subjectBiomarkers, Tumor
dc.subjectBiosensing Techniques
dc.subjectElectrochemical Techniques
dc.subjectElectrodes
dc.subjectHumans
dc.subjectImmunoassay
dc.subjectLimit of Detection
dc.subjectNeoplasms
dc.subjectPolymers
dc.subjectPyrroles
dc.subjectReceptors, CCR4
dc.subjectReproducibility of Results
dc.subjectTin Compounds
dc.titleFabrication of electrochemical immunosensor based on acid-substituted poly(pyrrole) polymer modified disposable ITO electrode for sensitive detection of CCR4 cancer biomarker in human serum
dc.typeArticle

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