Cytotoxic Antibody Detection by Means of Flow-Cytometric Cross-Match

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dc.authorid0000-0002-6688-2347
dc.authorid0000-0002-3015-0929
dc.authorscopusid9242258800
dc.authorscopusid42360894600
dc.authorscopusid57160120200
dc.authorscopusid6504396778
dc.authorscopusid8624948000
dc.authorwosidATA, PINAR/AAC-9967-2020
dc.authorwosidBilgen, Turker/N-6376-2018
dc.contributor.authorBilgen, Türker
dc.contributor.authorAta, P.
dc.contributor.authorTozkir, J.
dc.contributor.authorTozkır, Hilmi
dc.contributor.authorTitiz, Mesut İzzet
dc.date.accessioned2022-05-11T14:12:26Z
dc.date.available2022-05-11T14:12:26Z
dc.date.issued2017
dc.departmentYüksekokullar, Sağlık Yüksekokulu, Beslenme ve Diyetetik Bölümü
dc.departmentFakülteler, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Genel Cerrahi Ana Bilim Dalı
dc.description.abstractBackground. Complement-dependent lymphocytotoxicity (CDC-XM) and flowcytometric (FCXM) cross-match are analyzed individually for each donor and recipient pair, because these techniques have fundamental differences for the evaluation of histocompatibility. Lately, cytotoxic flow-cytometric cross-match (cFCXM) has been developed as an alternative to both CDC-XM and FCXM techniques. We evaluated the limits of cFCXM with the use of different positive serum dilutions. Methods. CDC-XM, FCXM, and cFCXM tests were performed with the use of commercially available negative and positive serum samples and lymphocytes from healthy donors. Results. Complement-dependent cell death was successfully detected with the use of cFCXM. Complement-dependent cell death ratios in cFCXM were similar those in CDCXM. With cFCXM, not only complement-dependent cell death but also IgG binding could be detected within a single assay. At higher concentrations of the positive serum, IgG-fluorescein isothiocyanate (FITC) mean fluorescent intensity (MFI) values detected with the use of cFCXM were less than those of conventional FCXM. Correspondingly, for dead cells, MFI values of IgG-FITC were less than those of live cells in higher positive serum concentrations in the cFCXM assay. Moreover, our results demonstrated that in cFCXM analysis, the decreasing ratio of dead cells at increasing positive serum dilutions was not in parallel with the same decrease in IgG-FITC MFI values. Conclusions. The cFCXM technique detects complement-mediated cytotoxic cell death with the additional ability to show IgG binding in the same tube and therefore may reduce the necessary bench time and workload.
dc.description.sponsorshipResearch Foundation Management Unit of Namik Kemal University [NKUBAP.02.GA.16.032]
dc.description.sponsorshipResearch Foundation Management Unit of Namik Kemal University (NKUBAP.02.GA.16.032).
dc.identifier.doi10.1016/j.transproceed.2017.01.016
dc.identifier.endpage444
dc.identifier.issn0041-1345
dc.identifier.issn1873-2623
dc.identifier.issue3en_US
dc.identifier.pmid28340808
dc.identifier.scopus2-s2.0-85015834796
dc.identifier.scopusqualityQ3
dc.identifier.startpage440
dc.identifier.urihttps://doi.org/10.1016/j.transproceed.2017.01.016
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5557
dc.identifier.volume49
dc.identifier.wosWOS:000398017900012
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorBilgen, Türker
dc.institutionauthorTitiz, Mesut İzzet
dc.language.isoen
dc.publisherElsevier Science Inc
dc.relation.ispartofTransplantation Proceedings
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectRenal-Transplantation
dc.subjectBinding
dc.subjectAssay
dc.subjectIgg
dc.titleCytotoxic Antibody Detection by Means of Flow-Cytometric Cross-Match
dc.typeArticle

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