The Mitochondrial DNA Control Region Might Have Useful Diagnostic and Prognostic Biomarkers for Thyroid Tumors

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dc.authorid0000-0002-6044-1372
dc.authorid0000-0001-5291-8620
dc.authorwosidsirin, duygu yasar/AAR-8685-2020
dc.authorwosidBircan, Rifat/A-7344-2018
dc.contributor.authorBircan, Rıfat
dc.contributor.authorIlıksu Gözü, Hülya
dc.contributor.authorUlu, Esra
dc.contributor.authorSarıkaya, Şükran
dc.contributor.authorGül, Aylin Ege
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorAral, Cenk
dc.date.accessioned2022-05-11T14:03:12Z
dc.date.available2022-05-11T14:03:12Z
dc.date.issued2019
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractThe literature suggests that mitochondrial DNA (mtDNA) defects are associated with a large number of diseases including cancers. The role of mtDNA variations in thyroid cancer is a highly controversial topic. Therefore, we investigated the role of mt-DNA control region (CR) variations in thyroid tumor progression and the influence of mtDNA haplogroups on susceptibility to thyroid tumors. For this purpose, in total, 108 hot thyroid nodules (HTNs), 95 cold thyroid nodules (CTNs), 48 papillary thyroid carcinoma (PTC) samples with their surrounding tissues and 104 healthy control subjects' blood samples were screened for all mtDNA CR variations using Sanger sequencing. We found that MtDNA haplogroup U was significantly associated with susceptibility to benign thyroid entities. In addition, eight single nucleotide polymorphisms (SNPs) (T146C, G185A, C194T, C295T, G16129A, T16304C, A16343G and T16362C) in the mtDNA CR were associated with the occurrence of benign and malign thyroid nodules in the Turkish population. As compared with samples taken from a healthy Turkish population and HTNs, the frequency of C7 repeats in D310 polycytosine sequence was found to be higher in CTNs and the PTC samples. In addition, the frequency of somatic mutations in mtMSI regions including T16189C and D514 CA dinucleotide repeats were found to be higher in PTC samples than benign thyroid nodules. Conversely, the frequency of somatic mutations in D310 was found to be higher in HTNs than CTNs and PTCs. In conclusion, mtDNA D310 instability does not play a role in the tumorigenesis of PTC but the results indicate that it might be used as a diagnostic clonal expansion biomarker for premalignant thyroid tumor cells. In addition, D514 CA instability might be considered as a prognostic biomarker for benign to malign transformation in thyroid tumors.
dc.description.sponsorshipResearch Fund of the Tekirdag Namik Kemal University [NKUBAP.00.10.AR.14.07, NKUBAP.00.10.AR.15.05]
dc.description.sponsorshipThis work was supported by two grants from the Research Fund of the Tekirdag Namik Kemal University, project numbers NKUBAP.00.10.AR.14.07 and NKUBAP.00.10.AR.15.05.
dc.identifier.doi10.1055/a-0869-7355
dc.identifier.endpage436
dc.identifier.issn0947-7349
dc.identifier.issn1439-3646
dc.identifier.issue7en_US
dc.identifier.pmid30986880
dc.identifier.startpage423
dc.identifier.urihttps://doi.org/10.1055/a-0869-7355
dc.identifier.urihttps://hdl.handle.net/20.500.11776/4638
dc.identifier.volume127
dc.identifier.wosWOS:000473537400002
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.institutionauthorBircan, Rıfat
dc.institutionauthorUlu, Esra
dc.institutionauthorŞirin, Duygu Yaşar
dc.institutionauthorAral, Cenk
dc.language.isoen
dc.publisherJohann Ambrosius Barth Verlag Medizinverlage Heidelberg Gmbh
dc.relation.ispartofExperimental And Clinical Endocrinology & Diabetes
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectmitochondrial DNA
dc.subjectcontrol region
dc.subjectD310
dc.subjectD514 CA repeat
dc.subjectT16189C
dc.subjectthyroid nodule
dc.subjectpapillary thyroid cancer
dc.subjectD-Loop Region
dc.subjectMolecular Pathogenesis
dc.subjectMutations
dc.subjectNodules
dc.subjectInstability
dc.subjectReceptor
dc.subjectIdentification
dc.subjectPolymorphisms
dc.subjectPopulation
dc.subjectEvolution
dc.titleThe Mitochondrial DNA Control Region Might Have Useful Diagnostic and Prognostic Biomarkers for Thyroid Tumors
dc.typeArticle

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