Effects of etanercept, a tumor necrosis factor receptor fusion protein, on primary cell cultures prepared from intact human intervertebral disc tissue

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dc.authorid0000-0002-8084-7855
dc.authorid0000-0001-8171-5929
dc.authorwosidsirin, duygu yasar/AAR-8685-2020
dc.authorwosidOzbek, Hanefi/O-3472-2019
dc.authorwosidAkyuva, Yener/AAV-5706-2020
dc.contributor.authorÇalışkan, Tezcan
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorÖzbek, Hanefi
dc.contributor.authorAkyuva, Yener
dc.contributor.authorAteş, Özkan
dc.contributor.authorŞimşek, Abdullah Talha
dc.date.accessioned2022-05-11T14:12:15Z
dc.date.available2022-05-11T14:12:15Z
dc.date.issued2019
dc.departmentFakülteler, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Beyin ve Sinir Cerrahisi Ana Bilim Dalı
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractThe aim of the present study was to investigate the effects of etanercept (ETA), a tumor necrosis factor (TNF) inhibitor, on human cell cultures prepared from intact intervertebral disc tissue. ETA is used as a treatment for cases of rheumatoid arthritis, psoriatic arthritis, axial spondyloarthritis and ankylosing spondylitis accompanied by moderate or severe joint pain. ETA was applied to primary cell cultures [annulus fibrosus and nucleus pulposus (NP) from intact intervertebral disc tissue]. Cell cultures without ETA treatment served as the control group. Morphological and quantitative molecular analyses of the two groups were performed. The number of viable cells and cell proliferation decreased in the ETA-treated cultures as compared with those in the control group. Furthermore, in the treatment group, the chondroadherin gene, an NP-specific marker, was not expressed after 24 h. By contrast, the cartilage oligo matrix protein was expressed 24, 48 and 72 h post-ETA treatment, while its expression was significantly lower than that in the control group. In addition, the expression of interleukin-1 beta, as well as matrix metallopeptidase-7 and -19, was markedly decreased. Overall, the cell proliferation and gene expression in the ETA-treated cells were significantly different from those in the control group (P<0.05). These results suggest that the treatment duration and dosage of TNF inhibitors, which are used to suppress active inflammation, should be considered in the clinical setting. These biological agents may delay the healing of intervertebral disc tissue damage by slowing cell proliferation and altering gene expression via anabolic and catabolic pathways.
dc.identifier.doi10.3892/etm.2019.7559
dc.identifier.endpage76
dc.identifier.issn1792-0981
dc.identifier.issn1792-1015
dc.identifier.issue1en_US
dc.identifier.pmid31258639
dc.identifier.startpage69
dc.identifier.urihttps://doi.org/10.3892/etm.2019.7559
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5464
dc.identifier.volume18
dc.identifier.wosWOS:000476606000009
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.institutionauthorÇalışkan, Tezcan
dc.institutionauthorKaraarslan, Numan
dc.institutionauthorŞimşek, Abdullah Talha
dc.institutionauthorŞirin, Duygu Yaşar
dc.language.isoen
dc.publisherSpandidos Publ Ltd
dc.relation.ispartofExperimental and Therapeutic Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcartilage oligomatrix protein
dc.subjectchondroadherin
dc.subjectetanercept
dc.subjectintact intervertebral disc tissue
dc.subjectinterleukin-1 beta
dc.subjectmatrix metalloproteinases
dc.subjectprimary cell culture
dc.subjectMatrix Metalloproteinases
dc.subjectNucleus Pulposus
dc.subjectGrowth-Factors
dc.subjectNitric-Oxide
dc.subjectFactor-Alpha
dc.subjectTnf-Alpha
dc.subjectDegeneration
dc.subjectExpression
dc.subjectInhibitor
dc.subjectPain
dc.titleEffects of etanercept, a tumor necrosis factor receptor fusion protein, on primary cell cultures prepared from intact human intervertebral disc tissue
dc.typeArticle

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