Novel zinc oxide nanoparticles of Teucrium polium suppress the malignant progression of gastric cancer cells through modulating apoptotic signaling pathways and epithelial to mesenchymal transition

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dc.authoridtemiz, cengiz/0000-0002-9265-7485
dc.authorwosidEge, Miray/AAU-7477-2020
dc.authorwosidtemiz, cengiz/AAZ-1310-2020
dc.contributor.authorBozgeyik, İbrahim
dc.contributor.authorEge, Miray
dc.contributor.authorTemiz, Ebru
dc.contributor.authorErdal, Berna
dc.contributor.authorKoyuncu, İsmail
dc.contributor.authorTemiz, Cengiz
dc.contributor.authorBozgeyik, Esra
dc.date.accessioned2023-05-06T17:23:36Z
dc.date.available2023-05-06T17:23:36Z
dc.date.issued2023
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalı
dc.description.abstractManagement of gastric cancer is still challenging due to resistance to current chemotherapeutics and recurrent disease. Moreover, green-synthesized zinc oxide nanoparticles (ZnO-NPs) using natural resources are one of the most promising therapeutic agents for anticancer therapy. Here we report the facile green synthesis and char-acterization of ZnO-NPs from Teucrium polium (TP-ZnO-NP) herb extract and the anticancer activities of these nanoparticles on gastric cancer cells. Facile green synthesis of TP-ZnO-NP was achieved using zinc acetate dihydrate. For the characterization of TP-ZnO-NP, UV-vis spectroscopy, FTIR, SEM, XRD and EDX analyses were performed. Antiproliferative and anticancer activities of TP-ZnO-NP were explored using the HGC-27 gastric cancer cell line model. MTT cell viability and colony formation assays were used for the analysis of cell pro-liferation and migration. Wound healing assay was used to analyze the migration capacities of cells. Annexin V/ PI double staining, DNA ladder assay, and Acridine orange/Ethidium bromide staining were performed to analyze the induction of apoptosis. qPCR was used to determine gene expression levels of apoptotic and epithelial to mesenchymal transition marker genes. The aqueous extract of TP served as both a reducing and capping agent for the successful biosynthesis of zinc oxide nanoparticles. Remarkably, synthesized TP-ZnO-NPs were found to have significant antiproliferative and anticancer activities on HGC-27 gastric cancer cells. Collectively, current data suggest that TP-ZnO-NP is a novel and promising anticancer agent for future therapeutic interventions in gastric cancer.
dc.identifier.doi10.1016/j.gene.2022.147091
dc.identifier.issn0378-1119
dc.identifier.issn1879-0038
dc.identifier.pmid36464168
dc.identifier.scopus2-s2.0-85143712874
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.gene.2022.147091
dc.identifier.urihttps://hdl.handle.net/20.500.11776/12181
dc.identifier.volume853
dc.identifier.wosWOS:000904587300003
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorErdal, Berna
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofGene
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAnticancer Agent
dc.subjectGastric Cancer
dc.subjectGreen Synthesis
dc.subjectNanoparticle
dc.subjectTeucrium Polium
dc.subjectGreen Synthesis
dc.subjectExtract
dc.subjectZno
dc.titleNovel zinc oxide nanoparticles of Teucrium polium suppress the malignant progression of gastric cancer cells through modulating apoptotic signaling pathways and epithelial to mesenchymal transition
dc.typeArticle

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