A new immunosensing platform based on conjugated Poly(ThidEp-co-EDOT) copolymer for resistin detection, a new obesity biomarker

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dc.contributor.authorAydin, Elif Burcu
dc.contributor.authorAydin, Muhammet
dc.contributor.authorSezginturk, Mustafa Kemal
dc.date.accessioned2024-10-29T17:58:18Z
dc.date.available2024-10-29T17:58:18Z
dc.date.issued2024
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractThe design of a novel electrochemical impedimetric biosensor for label-free analysis of resistin, a biomarker for obesity, is reported. For the fabrication of the immunosensor, a novel approach composed of electrochemical copolymerization of double epoxy groups-substituted thiophene (ThidEp) and 3,4-Ethylenedioxythiophene (EDOT) monomers was utilized. Anti-resistin antibodies were covalently attached to the copolymer-coated electrode. The capture of resistin antigens by anti-resistin antibodies caused significant variations in charge transfer resistance (R-ct) because of the immunoreactions between these proteins. Under optimum experimental variables, the changes in impedance signals were employed for the determination of resistin antigen concentration, and the prepared immunosensor based on conjugated copolymer illustrated a wide linear range between 0.0125 and 22.5 pg/mL, a low detection limit (LOD) of 3.71 fg/mL, and a good sensitivity of 1.22 k Omega pg(-1)mL cm(2). The excellent analytical performance of the resistin immunosensor in terms of selectivity, sensitivity, repeatability, reproducibility, storage stability, and low detection limit might be attributed to the conductive copolymer film layer generation on the disposable indium tin oxide (ITO) platform. The capability of this system for the determination of resistin in human serum and saliva samples was also tested. The immunosensor results were in accordance with the enzyme-linked immunosorbent assay (ELISA) results. The matrix effects of human serum and saliva were also investigated, and the proposed immunosensor displayed good recovery ranging from 95.91 to 106.25%. The engineered immunosensor could open new avenues for obesity monitoring.
dc.description.sponsorshipTUBIdot;TAK (The Scientific and Technological Research Council of Turkey)
dc.description.sponsorshipNo Statement Available
dc.identifier.doi10.1007/s00604-023-06145-8
dc.identifier.issn0026-3672
dc.identifier.issn1436-5073
dc.identifier.issue1en_US
dc.identifier.pmid38165489
dc.identifier.scopus2-s2.0-85181175844
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1007/s00604-023-06145-8
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14208
dc.identifier.volume191
dc.identifier.wosWOS:001137674500003
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer Wien
dc.relation.ispartofMicrochimica Acta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectResistin
dc.subjectObesity
dc.subjectElectrochemical immunosensor
dc.subjectModified electrode
dc.subjectSingle-frequency impedance
dc.subjectElectrochemical impedance spectroscopy
dc.subjectConjugated thiophene polymer
dc.titleA new immunosensing platform based on conjugated Poly(ThidEp-co-EDOT) copolymer for resistin detection, a new obesity biomarker
dc.typeArticle

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