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Öğe Evaluation of the relationship between serum ghrelin levels and cancer cachexia in patients with locally advanced nonsmall-cell lung cancer treated with chemoradiotherapy(Wolters Kluwer Medknow Publications, 2020) Uysal, Pelin; Usul Afşar, Çiğdem; Sözer, Volkan; İnanç, Berrin; Ağaoğlu, Fulya; Güral, Zeynep; Uzun, Hafize; Fazlıoğlu, NevinBackground: Ghrelin plays a role in mechanisms related to cancer progression - including cell proliferation, invasion and migration, and resistance to apoptosis in the cell lines from several cancers. We investigated the role of ghrelin levels in cancer cachexia-anorexia in patients with locally advanced nonsmall-cell lung cancer (NSCLC) treated with chemoradiotherapy (CRT). Materials and Methods: This study involved 84 NSCLC patients who had received concomitant CRT. Blood ghrelin levels were compared before and 3 months after CRT. Meanwhile, changes in body weight of the patients were also investigated with changes in ghrelin levels before and after CRT. Results: Ghrelin levels were significantly decreased in line with changes in patients' weights in patients receiving CRT (P < 0.001). Serum albumin levels and inflammatory-nutritional index were significantly decreased after radiotherapy (RT) (3.01 ± 0.40 g/dL, 0.38 ± 0.20) when compared with its baseline levels (3.40 ± 0.55 g/dL,P < 0.001; 0.86 ± 0.71,P < 0.001, respectively). Serum C-reactive protein levels were significantly increased after CRT (7.49 ± 6.53 mg/L) when compared with its baseline levels (9.54 ± 3.80 mg/L,P = 0.038). After RT, ghrelin levels in patients were positively correlated with body mass index (r = 0.830,P < 0.001) and albumin (r = 0.758,P < 0.001). Conclusion: Ghrelin may play a role in the pathogenesis of weight loss in NSCLC patients. Ghrelin seems to be implicated in cancer-related weight loss. Ghrelin, cancer, and RT all together have a role in tumor-related anorexia-cachexia in patients with NSCLC. Results of this study need further evaluation as regards to its potential role as an adjuvant diagnostic or prognostic marker. © 2020 Wolters Kluwer Medknow Publications. All rights reserved.Öğe Gestasyonel Diabetes Mellitusta Bazı Biyokimyasal Parametreler, Eser Element Düzeyleri Ve Lipid Peroksidasyonu Arasındaki İlişkiler(Namık Kemal Üniversitesi, Tıp Fakültesi, 2013) Kızıler, Ali Rıza; Aydemir, Birsen; Cinemre, F.Behice; Cinemre, Hakan; Gülyaşar, Tevfik; Tüten, Abdullah; Öncül, Mahmut; Açıkgöz, A.Serdar; Akdemir, Nermin; Erkorkmaz, Ünal; Korkmaz, Gülcan Güntaş; Uzun, HafizeAmaç Çalışmamızın amacı gestasyonel diabetes mellituslu (GDM) gebelerde ve sağlıklı gebelerde serum bakır (Cu), çinko (Zn), demir (Fe), rutin biyokimyasal parametreler ve plazma malondialdehit konsantrasyonlarının ölçümü ve bu parametreler arasındaki olası ilişkileri her iki grupta karşılaştırmaktır. Materyal ve Metod GDM olan 56 gebeden hasta grubu ile 60 sağlıklı gebeden oluşturulan kontrol grubunda plazma malondialdehit, kan şekeri, insülin, HbA1c, total kolesterol, trigliserit ve LDLkolesterol biyokimyasal yöntemlerle ölçüldü. Serum Fe, Cu ve Zn alev atomik absorpsiyon spektrofotometresi ile ölçüldü. Bulgular Kan şekeri, insülin, HOMA-IR, HbA1c, total kolesterol, LDL-kolesterol, plazma malondialdehit, serum Fe ve Cu konsantrasyonlarının GDM’li grupta kontrol grubuna göre anlamlı olarak yüksek olduğu saptandı. Ancak serum Zn konsantrasyonları GDM grubunda kontrol grubuna göre anlamlı olarak azaldığı görüldü. GDM hasta grubunda LDL-kolesterol ile serum Cu ve plazma malondialdehit arasında negatif bir korelasyon saptandı. Ancak serum Fe ile plazma malondialdehit arasında ise pozitif bir korelasyon olduğu görüldü. Sonuç Bulgularımız GDM’li hasta grubunda anlamlı olarak Zn düzeylerinin yetersizliğini, hiperglisemi, hiperlipidemi ve malondialdehitin artışını göstermektedir. Zn elementi takviyesi antioksidatif sistemi desteklemesi ile antioksidatif enzim aktivitesini arttırılarak fetal ve maternal komplikasyonlarda iyileşme beklenebilir.Öğe LOX-1 gene variants and maternal levels of plasma oxidized LDL and malondialdehyde in patients with gestational diabetes mellitus(Springer Heidelberg, 2016) Aydemir, Birsen; Baykara, Onur; Cinemre, Fatma Behice Serinkan; Cinemre, Hakan; Tüten, Abdullah; Kızıler, Ali Rıza; Uzun, HafizePurpose The aim of this study was to investigate the relationships between the maternal levels of oxidized LDL (ox-LDL), lipid peroxidation marker malondialdehyde (MDA) and LOX-1 3'UTR188C/T and K167N single nucleotide polymorphisms in pregnant Turkish women with gestational diabetes mellitus (GDM). Methods 116 pregnant women with GDM and 120 healthy pregnant women from the same geographic region were included in the study. Polymerase chain reaction-based restriction analysis was used to identify 3'UTR188C/T and K167N polymorphisms of the LOX-1 gene. Plasma ox-LDL and MDA levels were determined by enzyme-linked immunosorbent assay and spectrophotometric method in all study subjects, respectively. Results Our results indicated that the distribution of the LOX-1 3'UTR188C/T and K167N genotypes and alleles did not differ significantly among subjects with or without GDM (p> 0.05). TT and NN genotype carriers are associated with some glucose metabolism parameters (p< 0.05). There were no significant differences among plasma ox-LDL and MDA levels with regard to LOX-1 3'UTR188C/T and K167N polymorphisms in GDM group and control subjects (p> 0.05). According to the combined genotype analysis of LOX-1 3'UTR 188 TT and K167N NN polymorphisms, plasma MDA and ox-LDL levels were significantly different between women with GDM and healthy subjects either with or without combined TT/NN genotype carriers (p< 0.001). Conclusions According to our results, ox-LDL and MDA levels were increased in GDM pregnant women and healthy pregnant women either with or without combined TT/NN genotype carriers, for our Turkish sample, these genotype carriers appear to be related with increased oxidative stress in patients with GDM.Öğe Neuroprotective Effects of Darbepoetin Alpha on the Hippocampus in Experimental Ethanol Administration(Wiley, 2023) Uygur, Emine; Seymen, Hakkı Oktay; Uzun, Hafize; Tunçdemir, Matem; Öz, Aysim Buğe; Polat, Elif; Yılmaz, Ahsen[Abstract Not Available]Öğe Paraoxonase and Arylesterase Activities, Lipid Profile, and Oxidative Damage in Experimental Ischemic Colitis Model(Hindawi Ltd, 2012) Ünal, Ethem; Eriş, Cengiz; Kaya, Bülent; Uzun, Hafize; Çavdar, Faruk; Yıldar, Murat; Titiz, Mesut İzzet; Kızıler, Ali RizaObjective. In the present study, since PON1 is known as an HDL-associated antioxidant enzyme that inhibits the oxidative modification of LDL and oxidative stress plays a role in the pathogenesis of mesenteric ischemia, we investigated the changes in PON1 activity and lipid profile in an experimental ischemic colitis model. Methods. Forty male Wistar albino rats were divided into two groups: the control group (N = 15) and the experimental group (N = 25). All animals were anesthetized with ether and ketamine anesthesia to undergo a midline laparotomy. Ischemic colitis was induced by marginal vessel ligation in the splenic flexura (devascularization process). A sham laparotomy was performed in the control group. All animals were sacrificed on the seventh postoperative day. Oxidative stress marker (malonyldialdehyde, MDA), lipid profile, and paraoxonase (PON-1) and arylesterase activities were determined. Histopathological evaluation was done under light microscopy, after sectioning and staining with hematoxyline and eosin. Statistical analysis was conducted using Student's t-test and Mann-Whitney U test, and P < 0.05 was considered as statistically significant. Results. There was a significant decrease in both serum and tissue PON1 activity in ischemic colitis group (P < 0.01, for each). Similarly, arylesterase levels showed a parallel decrease in both tissue and serum of the experimental group (P < 0.01 and P < 0.001, retrospectively). MDA, an oxidative stress marker, was seen to increase in the experimental group (P < 0.01, tissue; P < 0.05, serum). In experimental group, there was a significant rise in serum total cholesterol and LDL levels (P < 0.001, for each). However, HDL level decreased significantly (P < 0.001). Triglycerides did not show any change between the groups (P > 0.05).Conclusions. PON1 and arylesterase play an important role in the pathophysiology of ischemic colitis.Öğe Serum paraoxonase (a high-density lipoprotein-associated lipophilic antioxidant) activity in clinical follow-up of patients with acute pancreatitis, with particular emphasis on oxidative stress parameters and lipid profile: a prospective pilot trial(Taylor & Francis Ltd, 2019) Yüksekdağ, Sema; Yüksel, Esra; Topçu, Ahmet; Karaağaç, Neslihan; Uzun, Hafize; Kızıler, Ali Rıza; Ünal, EthemThe purpose of this study was to investigate the possible role of PON-1, an antioxidant lipophilic enzyme linked to HDL-C (high-density lipoprotein cholesterol), on the pathophysiology and clinical follow-up of acute pancreatitis. Biochemical tests, PON-1 and oxidative stress parameters (malonyl dialdehyde, MDA; superoxide dismutase, SOD; total antioxidant capacity, TAC) were evaluated in the sera of patients with acute pancreatitis at admission (day 0), day 3 and day 10 of follow-up, between June and September 2017. SPSS 13.0 statistical software package programme was used for statistical analyses.Mean age was 51.4 of the total 25 patients. Ranson scores were 0-1 points (60%), 3-4 points (24%) and 5-6 points (16%). CTSI (computed tomography severity index) scores were calculated, and most of the patients were seen to have mild or average pancreatitis (96%). While total cholesterol, triacylglycerol and LDL-C (low-density lipoprotein) levels stayed in their normal limits, there was a significant decrement tendency. HDL-C level was seen to rise significantly above its upper limit at day 10 (p < 0.001). Mean PON-1 levels were measured as 69.23, 76.72 vs. 113.15 U/mL at days 0, 3 and 10, respectively; and it was positively correlated with HDL-C (p < 0.001). Serum SOD increased also in parallel with PON-1 (20.49 vs. 39.46 U/mL) while MDA level decreased significantly (3.9 vs. 2.28 mu M, p < 0.001). TAC was seen to rise significantly after treatment (0.52 vs. 1.22 mM). In conclusion, decreased PON-1 and HDL-C together with antioxidants SOD and TAC at the early period of acute pancreatitis were seen to rise after treatment, while the previously higher MDA level decreased in parallel. This reveals the importance of the balance between oxidative stress and antioxidant defense mechanisms in clinical progression of the disease, and the potential of PON-1 as a promising clinical marker.Öğe Significance of Plasma Irisin, Adiponectin, and Retinol Binding Protein-4 Levels as Biomarkers for Obstructive Sleep Apnea Syndrome Severity(Mdpi, 2023) Fazlioglu, Nevin; Uysal, Pelin; Durmus, Sinem; Yurt, Sibel; Gelisgen, Remise; Uzun, HafizeObjective: Obstructive sleep apnea syndrome (OSAS) is a common sleep disorder that is caused by the reduction or cessation of airflow in the upper airway. Irisin, retinol-binding protein-4 (RBP-4), and adiponectin are the three significant factors in the metabolic process of the human body. The objective of this study was to investigate whether plasma irisin, RBP-4, and adiponectin levels are associated with the severity of OSAS. Methods: According to inclusion and exclusion criteria, 125 patients with OSAS and 46 healthy, gender-matched controls were included in this study. The patients were classified according to the apnea hypopnea index (AHI) as 14 mild cases (5 < AHI < 15), 23 moderate OSAS cases (15 < AHI < 30), and 88 severe OSAS cases (AHI > 30). The plasma irisin, RBP-4, and adiponectin levels were measured and compared between groups. Results: RBP-4 levels were higher in severe OSAS compared to other groups, and irisin levels were significantly lower in severe OSAS compared to other groups. There was a negative correlation between irisin and RBP-4 (r = -0.421; p < 0.001), and irisin and AHI (r = -0.834; p < 0.001), and a positive correlation between irisin and adiponectin (r = 0.240; p = 0.002). There was a negative correlation between RBP-4 and adiponectin (r = -0.507; p < 0.001) and a positive correlation between RBP-4 and AHI (r = 0.473; p < 0.001). As a predictor of OSAS, adiponectin showed the highest specificity (84.8%) and RBP-4 the highest sensitivity (92.0%). Conclusion: Circulating adiponectin, irisin, and RBP-4 may be new biomarkers in OSAS patients in addition to risk factors such as diabetes, obesity, and hypertension. When polysomnography is not available, these parameters and clinical data can be used to diagnose the disease. As a result, patients with an AHI score greater than thirty should be closely monitored for metabolic abnormalities.Öğe The association of lectin-like oxidized LDL receptor 1 (LOX-1) K167N and 3'UTR188CT polymorphisms with maternal plasma soluble LOX-1 levels and preeclampsia risk in Turkish population(Springer Heidelberg, 2015) Tüten, Abdullah; Aydemir, Birsen; Öncül, Mahmut; Kızıler, Ali Rıza; Açıkgöz, A.Serdar; Güntaş Korkmaz, Gülcan; Uzun, HafizeTo investigate the main effect of polymorphisms in genes involved in endothelial pathophysiological mechanisms, LOX-1 K167N and 3'UTR188CT single nucleotide polymorphisms (SNPs) in relation to preeclampsia (PE) risk and possible interactions between the gene polymorphisms and plasma oxLDL and soluble LOX-1 (sLOX-1) levels on PE in Turkish population. LOX-1 K167N and 3'UTR188CT polymorphisms were studied in 113 pregnant women with preeclampsia and 96 healthy pregnant women by the PCR-RFLP techniques. sLOX-1 and oxLDL levels were determined by enzyme-linked immunosorbent assay (ELISA) in all study subjects. Patients having LOX-1 3'UTR188CT (OR 3.55, 95 % CI 1.89-6.67, P = 0.001) or 3'UTR188CC (OR 3.04, 95 % CI 1.25-7.38, P = 0.012) genotype had a significantly higher risk of PE than those with 3'UTR188TT genotype. Also, patients having K167N KK (OR 2.73, 95 % CI 1.33-5.61, P = 0.005) genotype had a significantly higher risk of PE than those with K167N NN genotype. LOX-1 3'UTR188TT and LOX-1 K167N NN genotype carriers were associated with significantly increased serum sLOX-1 level (P = 0.001). We further investigated the potential combined effect of these polymorphic variants on risk of PE development. According to the combined genotype analysis of LOX-1 3'UTR188TT and K167N NN polymorphisms, sLOX-1 and oxLDL levels also showed significant differences between PE patients and controls with or without combined TT/NN genotype carriers. Our findings indicate that higher plasma sLOX-1 and oxLDL concentrations, and the LOX-1 3'UTR188C > T and LOX-1 K167N gene polymorphisms were significantly associated with risk of developing preeclampsia. Plasma sLOX-1 may be a potential therapeutic target in the treatment of preeclampsia.Öğe The Effects of Darbepoetin Alfa on the Changes Induced by Ethanol in Glucose Metabolism and Lipid Profile(Wiley, 2023) Uygur, Emine; Seymen, Hakkı Oktay; Uzun, Hafize; Tunçdemir, Matem; Öz, Aysim Buğe; Polat, Elif; Yılmaz, Ahsen[Abstract Not Available]Öğe The effects of hyperbaric oxygen application against cholestatic oxidative stress and hepatic damage after bile duct ligation in rats(Academic Press Inc Elsevier Science, 2013) Ayvaz, Süleyman; Kanter, Mehmet; Aksu, Burhan; Şahin, Sevtap Hekimoglu; Uzun, Hafize; Erboğa, Mustafa; Pul, MehmetBackground: The aim of this study was to evaluate the preventive and therapeutic potential of hyperbaric oxygen therapy (HBO) on the liver tissue against bile duct ligation (BDL)-induced oxidative damage and fibrosis in rats. Materials and methods: We divided 32 adult male Sprague Dawley rats into four groups: sham, sham plus HBO, BDL, and BDL plus HBO; each group contained eight animals. We placed the sham plus HBO and BDL plus HBO groups in an experimental hyperbaric chamber in which we administered pure oxygen at 2.5 atmospheres absolute 100% oxygen for 90 min on 14 consecutive days. Results: The application of BDL clearly increased the tissue malondialdehyde level, myeloperoxidase activity, and hydroxyproline content and decreased the antioxidant enzymes (superoxide dismutase and catalase activities) and glutathione level. Hyperbaric oxygen therapy treatment significantly decreased the elevated tissue malondialdehyde level, myeloperoxidase activity, and hydroxyproline content and increased the reduced superoxide dismutase and catalase activities and glutathione level in the tissues. The changes demonstrating the bile duct proliferation and fibrosis in expanded portal tracts include the extension of proliferated bile ducts into lobules, mononuclear cells, and neutrophil infiltration into the widened portal areas were observed in BDL group. Treatment of BDL with HBO attenuated alterations in liver histology. Alpha smooth muscle actin, cytokeratinpositive ductular proliferation, and the activity of terminal deoxynucleotidyl transferase 2'-deoxyuridine, 5'-triphosphate nick end labeling in the BDL decreased with HBO treatment. Conclusions: The data indicate that HBO attenuates BDL-induced oxidative injury, hepatocytes damage, bile duct proliferation, and fibrosis. The hepatoprotective effect of HBO is associated with antioxidative potential. (C) 2013 Elsevier Inc. All rights reserved.
