Yazar "Muz, Dilek" seçeneğine göre listele
Listeleniyor 1 - 15 / 15
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe A molecular epidemiological study of black queen cell virus in honeybees (Apis mellifera) of Turkey: the first genetic characterization and phylogenetic analysis of field viruses(Springer France, 2018) Muz, Dilek; Muz, Mustafa NecatiBlack queen cell virus (BQCV) is one of the most common honeybee pathogens causing queen brood deaths. The 63 apiaries were sampled between 2007 and 2013 from four different ecogeographic regions in Turkey to estimate BQCV molecular structural characteristics. The BQCV positivity was 47.6%. The 25 local Black queen cell viruses (TrBQCVs) were molecularly characterized and investigated for their genetic relationship with previous records. The identity of the helicase gene among the TrBQCVs was 92-98%, whereas the similarity ranged from 37 to 85% in comparison with the intercontinental records. The identity of the partial capsid gene among the TrBQCVs was 91-100%, and the similarity rate varied from 86 to 97, 88-96, 90-97 and 89-99% in comparison with the Asian, African, American and European counterparts, respectively. The four nonsynonymous substitutions on the partial capsid protein suggest a predicted genotype that is specific among TrBQCVs.Öğe Acute bee paralysis virus field isolates from apiaries suffering colony losses in Turkiye(Taylor & Francis Ltd, 2023) Muz, Dilek; Muz, Mustafa NecatiMultiple viral infections are one of the main drivers of honey bee colony losses and threaten the sustainability of colony health. The Acute bee paralysis virus (ABPV) is one of the common honey bee viruses that can induce overt and/or covert infections in bee colonies. Globally more knowledge is needed about its effects leading to colony losses. ABPV-related pathology includes paralytic, trembling bees, unable to fly and, in some cases, ABPV causes the death of the bee colonies. The molecular phylogenetic of ABPV isolates obtained from apiaries suffering colony losses and/or with CCD-like complaints in Turkiye was performed in this study. The isolates' structural and non-structural gene regions were analyzed phylogenetically and compared according to sampling years and apiaries. According to the results, the nucleotide identity of the RNA-dependent RNA polymerase (RdRp) gene in Turkish ABPV field isolates is more conserved than the capsid gene. The evaluation of molecular differentiation among the ABPV field isolates can be used for determining the origin and relatedness of ABPV strains and periodic monitoring of ABPV infections in apiaries.Öğe Apoptosis in cancer(2022) Yılmaz, Melisa Beyhan; Muz, DilekApoptosis is a programmed cell death mechanism, with its presence and absence, directs the progression of most diseases and is targeted in disease treatments. Many factors are influential in the cell's pathway to apoptosis. The defects in these pathways may transform the cell become malignant, and the organism may face a lethal outcome such as cancer. Understanding apoptosis will provide clues in guiding the pathogenesis of diseases. Two main pathway leading to apoptosis, intrinsic and extrinsic, take an active role. Another route is activated by enzymes and enzymes secreted by immune cells such as T and NK. With their initiator and enforcer roles, many caspase molecules are active at critical points in the cell's apoptosis process. In cancer treatments, activation of molecules in these pathways and repair of disrupted pathways are among the target approaches. This review discuss target strategies for inhibiting apoptotic pathways and molecules in cancer cells and the activation of these apoptotic pathways.Öğe Detection of Feline Coronavirus, Feline Immunodeficiency Virus, Feline Leukemia Virus, and Other Pathogen Genetic Material in Whole Blood From Domestic Cats in Turkiye(Aves, 2023) Muz, Dilek; Muz, Mustafa NecatiMany pathogens threaten feline health at all ages. Some pathogens suppress the immune system in cats, induce immunodeficiency, and predispose cats to other pathogens. In this study, 150 clinically sick (n = 119) and healthy-looking (n = 31) cats presented to private veterinary clinics with suspicious feline infectious peritonitis were sampled. Feline coronavirus, feline calicivirus, feline herpesvirus-1, feline immunodeficiency virus, feline leukemia virus, parvovirus, and Anaplasma species were investigated using pathogen-specific polymerase chain reaction protocols. Analysis results and demographic information of cats were evaluated. According to results, the positivity rates of feline coronavirus, feline leukemia virus, Anaplasma sp., feline immunodeficiency virus, parvovirus, feline calicivirus and feline herpesvirus were detected as 23.3%, 54%, 46%, 26%, 6.7%, 6% and 2.7%, respectively. The 11 cats (7.3%) were defined for wet feline infectious peritonitis. This research is the first report regarding feline herpesvirus-1, feline calicivirus, and parvovirus infections in cats in Tekirdag province. The results showed that viral agents and co-infections are common in domestic cats. Pathogen positivity was higher at younger ages and in clinically sick cats. The possibility of the coexistence of multiple pathogens in cats applying to the clinics should be considered for treatment success and the regulation of vaccination programs.Öğe Expression and production of recombinant proteins from immunodominant E gene regions of bovine viral diarrhoea virus 1 (BVDV-1) Turkish field strains for prophylactic purpose(Ecole Nationale Veterinaire Toulouse, 2017) Oğuzoğlu, T. C.; Muz, Dilek; Timurkan, M. O.; Koç, B. T.; Özşahin, E.; Burgu, I.; Demirbağ, Z.Since the first description of Bovine Viral Diarrhoea Virus (BVDV) infection, many studies have been performed, and control and eradication programs were developed worldwide. Several studies have been conducted on the presence/prevalence and molecular characterization of BVD viruses in Turkey. However, there is no effective struggle programs for BVDV infection up to now in Turkey. Current study has been conducted for the identification of the E gene region which encodes several immunogenic proteins of BVDV, tested the immunogenicity of the recombinant proteins for possible vaccine production. We focused on the molecular characterization of the E gene region of field pestivirus isolates (n=40) which were obtained in Turkey between 1997 and 2007. Two recombinant proteins, E-ms and E2, were chosen from the E region and expressed in baculovirus vector system. The acquired proteins (approximately 34 kDa for E-ms belonging to BVDV 1-a and 44 kDa for E2 belonging to BVDV 1-l) triggered a humoral immune response in mice. These proteins could represent a good starting material in preparation of vaccines which are indispensable for control and eradication programs of pestivirus infections in Turkey.Öğe ISRAEL ACUTE BEE PARALYSIS VIRUS PREVALENCE IN APIARIES WITH COLONY LOSS IN TÜRKİYE(Bursa Uludag University, 2023) Muz, Dilek; Muz, Mustafa NecatiHoneybees are indispensable pollinator insects for vegetative pollination and biodiversity. Moreover, they serve medicinal importance with products such as honey, propolis, pollen, and royal jelly. Sudden bee deaths and colony collapse disorder (CCD) threaten the sustainability of colony health. Honeybee viruses, parasites, and pathogens trigger colony losses and CCD. This study investigated the presence and prevalence of Israeli acute bee paralysis virus (IAPV) in apiaries with sudden bee deaths, colony losses, and CCD-like problems in 16 provinces in different eco-geographic regions of Türkiye between 2011- 2021. Samples were tested for the coexistence of honeybee pathogens with IAPV. The sampled apiaries were evaluated for other bee pathogens such as Acute bee paralysis virus, Black queen bee virus, Chronic bee paralysis virus, Deformed wing virus, Kashmir bee virus, Lake Sinai virus, Sacbrood virus, Varroa mites, and Nosema sp. analyzed. Pathogen-specific RT-PCR assay was used for bee viruses. IAPV positivity was found to be 52.5% in apiaries. 97.5% of the sampled apiaries were positive for at least one pathogen. According to the results of this study, the presence of IAPV in apiaries suffering from colony loss and CCD-like problems was higher than in previous reports, and viruses of different species, Nosema sp., and varroa infestation were found to be frequently encountered. The results suggest that the coexistence of IAPV and multiple pathogens may be effective in colony losses. © 2023 The Author(s).Öğe Myxoma Virus Combination Therapy Enhances Lenalidomide and Bortezomib Treatments for Multiple Myeloma(Mdpi, 2024) Yesilaltay, Alpay; Muz, Dilek; Erdal, Berna; Bilgen, Turker; Batar, Bahadir; Turgut, Burhan; Topcu, BirolThis study aimed to explore the effectiveness and safety of Myxoma virus (MYXV) in MM cell lines and primary myeloma cells obtained from patients with multiple myeloma. Myeloma cells were isolated from MM patients and cultured. MYXV, lenalidomide, and bortezomib were used in MM cells. The cytotoxicity assay was investigated using WST-1. Apoptosis was assessed through flow cytometry with Annexin V/PI staining and caspase-9 concentrations using ELISA. To explore MYXV entry into MM cells, monoclonal antibodies were used. Moreover, to explore the mechanisms of MYXV entry into MM cells, we examined the level of GFP-labeled MYXV within the cells after blocking with monoclonal antibodies targeting BCMA, CD20, CD28, CD33, CD38, CD56, CD86, CD117, CD138, CD200, and CD307 in MM cells. The study demonstrated the effects of treating Myxoma virus with lenalidomide and bortezomib. The treatment resulted in reduced cell viability and increased caspase-9 expression. Only low-dose CD86 blockade showed a significant difference in MYXV entry into MM cells. The virus caused an increase in the rate of apoptosis in the cells, regardless of whether it was administered alone or in combination with drugs. The groups with the presence of the virus showed higher rates of early apoptosis. The Virus, Virus + Bortezomib, and Virus + Lenalidomide groups had significantly higher rates of early apoptosis (p < 0.001). However, the measurements of late apoptosis and necrosis showed variability. The addition of MYXV resulted in a statistically significant increase in early apoptosis in both newly diagnosed and refractory MM patients. Our results highlight that patient-based therapy should also be considered for the effective management of MM.Öğe Oncolytic Myxoma virus Increases Autophagy in Multiple Myeloma(Galenos Publ House, 2023) Yesilaltay, Alpay; Muz, Dilek; Erdal, BernaObjective: Multiple myeloma, which affects plasma cells, is the second most common hematological malignancy. Despite the development of new drugs and treatment protocols, patient survival has not reached the desired level. In this study, we investigated the effects of Myxoma virus (MYXV), an oncolytic virus, on autophagy in myeloma cells. Materials and Methods: We analyzed protein expressions of ATG-5, p62, Beclin-1, LC3B, and the apoptosis marker Bcl-2 as autophagy markers in human U-266 and mouse MOPC-315 myeloma cell lines subjected to different doses of MYXV. In addition, autophagic images of myeloma cells were investigated using transmission electron microscopy (TEM). Results: In the first 24 h, which is the early stage of autophagy, ATG-5 and Beclin-1 expression levels were increased in the U-266 and MOPC315 cell lines in the groups that had received MYXV at a multiplicity of infection of 15. At 48 h, a significant increase was detected in the expression of LC3B, which is a late indicator. Autophagosomes were observed in myeloma cells by TEM. Conclusion: MYXV shows an antimyeloma effect by increasing autophagy in myeloma cells.Öğe Tekirdağ'da "Koloni Kaybı Sendromu" Benzeri Kayıp Görülen Arılıklarda Bazı Patojenlerinin Araştırılması(2017) Muz, Dilek; Muz, Mustafa NecatiDünya'da on yıldan fazla süredir dikkat çeken koloni kayıpları, farklı etken ve faktörlerin etkileşimi ile ortaya çıkan arı sağlığı sorunu olarak kabul edilmektedir. Bal arısı koloni sağlığını tehdit eden hastalık etkenleri arasında viruslar, Nosema ceranae ve Varroa destructor ciddi öneme sahiptir. Bu çalışmada 2015 ve 2016 yıllarında Tekirdağ ilindeki farklı arılıklardan gönderilen balarısı numuneleri incelenmiştir. Örnekler "koloni populasyonundaki beklenmeyen azalma veya ani koloni kaybı" şikâyetleriyle gönderilmiştir. Buna göre 17 arılıktan gönderilen 510 bal arısı örneği, Deforme kanat virusu (Deformed Wing Virus - DWV), Nosema cerenae ve Varroa destructor yönünden kontrol edilmiştir. Bu arılıkların tamamında varroosis tespit edilirken, DWV 15 arılıkta, N. ceranae ise 5 arılıkta tespit edilmiştir. DWV tespit edilemeyen iki arılıkta N. ceranae da tespit edilememiştir. Bu araştırmada, ani koloni kaybı sendromuna benzer şekilde görülen koloni kayıplarında DWV, Nosema cerena ve Varroa destructor'a rastlanma oranlarının araştırılarak bu konuda güncel veri sağlanması hedeflenmiştir.Öğe The Genetic Characterization of Lake Sinai Virus in Colony Losses Apiaries in Türkiye(2024) Muz, Dilek; Muz, Mustafa NecatiObjective: Honeybees (Apis mellifera) have a unique role in natural pollination and maintaining biodiversity in the ecosystem. The alarming increase in unexpected colony losses, mysterious bee deaths, and the tragic extinction of entire colonies (Colony collapsed disorder- CCD) have sounded a global alarm, demanding immediate attention and collaborative action to address these critical challenges in bee breeding. Diseases, parasites, and pathogens significantly threaten colony health. Türkiye is a significant honey producer, providing an ideal environment for beekeeping due to its unique eco-geographical features. Unexpected colony losses and bee deaths are also questions of concern for beekeepers in Türkiye. Material Method: In this study investigated honey bee viruses in apiaries experiencing sudden bee death losses and CCD-like symptoms between 2021 and 2023 in Türkiye, involving genetic analysis of the LSV RdRp gene region. The honeybee and varroa samples were obtained from 52 colonies in 26 apiaries complaining of unexpected bee deaths and CCD-like symptoms between May 2021 and September 2023. Result: The results showed a high DWV, BQCV, and LSV prevalence, respectively. The sampled apiaries were infested mild-moderate- high grade with Varroa mites. Following PCR results, DWV, BQCV, LSV, IAPV, CBPV, and SBV positivity was detected at 69.2% (n=18), 50% (n=13), 38.46% (n=10), 26.9% (n=7), 19.2% (n=5) and 3.8% (n=1), respectively. High rates of multiple virus coexisting and high varroa infestation were noted in colonies with heavy losses and CCD-like complaints. The RdRp gene from two LSV samples (TrLSV-6474, TrLSV-6517) was sequenced. Turkish LSV samples (TrLSVs) showed a 72.88% homology of each other and clustered LSV4 branches in the phylogenetic tree. Turkish LSV sequences showed a closer similarity rate than reference sequences in GenBank with Asian Korean, Chinese, and Japanese LSV sequences. Conclusion: Further investigation is needed to comprehend the implications of elevated LSV populations on colony losses. The execution of genetic research with a more extensive sample size can significantly enhance the demonstration of species diversity and provide valuable insights into the influence of LSV variants on honeybee health and the management of diseases.Öğe The geographical distribution and first molecular analysis of Culicoides Latreille (Diptera: Ceratopogonidae) species in the Southern and Southeastern Turkey during the 2012 outbreak of bovine ephemeral fever(Springer, 2014) Dik, B.; Muz, Dilek; Muz, Mustafa Necati; Uslu, U.This study investigated the geographical distribution and molecular analysis of Culicoides species in the Southern and Southeastern Turkey during the 2012 outbreak of bovine ephemeral fever (BEF). The midge specimens caught by Onderstepoort-type light traps from livestock farms were tested for molecular evidence of existence of viral genome. Blood specimens were collected from clinically BEF-suspected acute febrile cattle. Total nucleic acid samples obtained from field specimens were checked against the BEF virus G gene and Culicoides internal transcribed spacer 1 (ITS-1) gene. A total of 20,845 Culicoides specimens (20,569 a (TM) Euroa (TM) Euro, 276 a (TM),a (TM),) comprising 11 species (Culicoides badooshensis, Culicoides circumscriptus, Culicoides gejgelensis, Culicoides imicola, Culicoides kibunensis, Culicoides longipennis, Culicoides newsteadi, Culicoides nubeculosus, Culicoides odiatus, Culicoides punctatus, Culicoides schultzei, Culicoides spp.) were collected. C. schultzei (18,032) was found as the dominant species and followed by C. imicola (1,857), C. nubeculosus complex (545), and C. circumscriptus (259), respectively. C. kibunensis was identified as new species for this region. PCR positivity of BEF was found 37.14 % (13/35) in blood samples whereas no viral genome was obtained from Culicoides specimens. Culicoides spp. ITS-1 gene sequences were analyzed phylogenetically with GenBank ITS-1 sequences. Molecular homology of Culicoides ITS-1 gene was ranged between 62.74 and 71.39 %. The results described first molecular detection and phylogenetic analysis of Culicoides ITS-1 gene with reference to the 2012 BEF outbreak in Turkey.Öğe The investigation of Culicoides (Diptera: Ceratopogonidae) species and Bluetongue virus and Schmallenberg virus in Northwest Turkiye(Springer, 2023) Muz, Dilek; Dik, Bilal; Muz, Mustafa NecatiCulicoides biting midges (Diptera: Ceratopogonidae) act as mechanical and biological vectors of arboviruses and are crucial in the global spread of these viruses. This study investigated the diversity of distribution of Culicoides species and the presence of Bluetongue virus (BTV) and Schmallenberg virus (SBV) in Tekirdag province in Northwest Turkiye. The fourteen Culicoides species, such as Culicoides newsteadi, Culicoides schultzei, Culicoides nubeculosus comp., Culicoides punctatus, Culicoides circumscriptus, Culicoides obsoletus comp., Culicoides gejgelensis, Culicoides festivipennis, Culicoides longipennis, Culicoides spp., Culicoides pulicaris, Culicoides picturatus, Culicoides odiatus, Culicoides kurensis, and Culicoides flavipulicaris, were detected. Culicoides newsteadi, C. odiatus, and C. pulicaris were the most abundant species. Phylogenetic analyses of Culicoides species' ITS-1 gene region were performed. A pool of C. festivipennis was positive for SBV RNA, while the BTV genomic materials was not found in the qPCR analysis. This is the first report of the presence/detection of SBV in Culicoides species in Turkiye. The survey of bioecological and epizootiological aspects of vector species is essential in implementing effective control measures for arboviral infections.Öğe The molecular and serological investigation of Feline immunodeficiency virus and Feline leukemia virus in stray cats of Western Turkey(Elsevier Ltd, 2021) Muz, Dilek; Can, Hamit; Karakavuk, Muhammet; Döşkaya, M.; Özdemir, H.G.; Değirmenci Döşkaya, A.; Muz, Mustafa NecatiThis study aimed to investigate the Feline immunodeficiency virus (FIV) / Feline leukemia virus (FeLV) infection prevalence among looking healthy stray cats in Western Turkey by serologic and molecular-based tests. A total of 1008 blood samples from the stray cats were used in this study. All samples were tested for FIV antibodies / proviral DNA and FeLV antibodies / antigens / proviral DNA. The genetic characterization and phylogenetic analysis of FeLV and FIV were carried out in this study. These cats also tested for Leishmaniasis and Toxoplasmosis previously. FIV Ab and proviral DNA detected in 25.2 % and 25.5 % of samples, respectively. FeLV Ab, Ag, proviral DNA positivity was in 45.2 %, in 3.3 %, in 69.7 %, respectively. The molecular detection and phylogenetic analysis of the current FeLV pol gene and FIV gag gene performed. The molecular characterization for the pol gene of FeLV (enFeLV and exFeLV) among Turkey's cat population was reported for the first time. The exFeLV pol sequences closer to the FeLV-A genotype, and the enFeLV pol sequences overlapped with other enFeLV. The current FIV gag sequences were clustered within the subtypes A, B, and C. The findings revealed FeLV subtype A and FIV subtype-A, subtype-B, subtype-C circulate among Turkish stray cats. Single and multiple co-infection positivity was found higher compared to previous reports. © 2021 Elsevier LtdÖğe The molecular prevalence of Borrelia burgdorferi, Babesia spp., and Anaplasma spp. in shelter dogs of the Thrace Region in Turkey(Scientific Technical Research Council Turkey-Tubitak, 2022) Altuğ, Nuri; Muz, Mustafa Necati; Muz, Dilek; Yipel, Fulya AltınokThe study aimed to update the molecular prevalence of some tick-borne pathogens (Borrelia burgdorferi, Babesia spp., and Anaplasma spp.) in the shelter dogs of the Thrace Region, Turkey. The study was carried out on 450 dogs from 7 pet shelters. The individual data of the dogs were recorded, and blood samples were collected in tubes with anticoagulants (EDTA). Then, individual PCR protocols were applied to all samples for the three infective agents. PCR test results recorded for B. burgdorferi is 38.22% (n = 172), 24.22% (n = 109) for Babesia spp., and 21.6% (n = 97) for Anaplasma spp. The positivity of dogs with at least one pathogen was 56.22% (n = 253). Only one pathogen positivity rate was determined in positive samples as 56.92% (n = 144). The positivity was determined 33.99% (n = 86) for two pathogens and 9.09% (n = 23) for three pathogens. The coexistence of the two pathogens was statistically significant (p < 0.01). The effect of sex and age was not statistically significant in the agent positivity (p < 0.01, p < 0.05). Among tested three pathogens, only the positivity of B. burgdorferi (p = 0.155) was statistically significant compared with the prevalence of the others (p < 0.01). As a result, pathogens transmitted by ticks in shelter dogs of the Thrace region were simultaneously investigated and detected for the first time. Results revealed that shelter dogs pose a hidden risk for animal and human health in the region and so the necessity to plan systematic epidemiological studies about tick-borne zoonose pathogens more frequently.Öğe VETERİNER FAKÜLTESİ ÖĞRENCİLERİNİN BAZI UYGULAMA DERSLERİ İLE İLGİLİ GÖRÜŞLERİ: ANKET ÇALIŞMASI(Balıkesir Üniversitesi, 2018) Altuğ, Nuri; Özdemir, Nurullah; Muz, Dilek; Seyidoğlu, Nilay; Can, Mehmet Ferit; Erdoğan, Serkan; Muz, Mustafa NecatiGİRİŞ: Bu araştırmada Veteriner Fakültesi öğrencilerinin bazı uygulama derslerindeki laboratuvar olanaklarının, eğitimin kalitesi, öğrenci motivasyonu ve memnuniyet düzeylerine olan etki ve katkılarının belirlenmesi amaçlandı YÖNTEM: Araştırma 29’u bayan 36’sı erkek toplam 65 öğrenci ile gerçekleştirildi. Öncelikle, Anatomi uygulamalarında plastik modelleri (1. ve 2. Sınıf), Fizyoloji uygulamalarında elektrokardiyografi (2. sınıf), laboratuvar uygulamalarında (1. ve 2. Sınıf) seroloji ve moleküler yöntemleri içeren uygulamalı dersler verildi. Uygulama derslerindeki materyallerinin yeterliliği, öğrenci eğitimlerine etkisi ve mesleki gelişime katkısı anket ile değerlendirildi. BULGULAR: Araştırma sonucunda laboratuvar kullanımı ve katkısıyla ilgili memnuniyet düzeyi %73,4 oranı ile “iyi” olarak belirlendi. Öğrencilerin %93,8’i seroloji ve moleküler yöntemleri içeren uygulama derslerinin arttırılması gerektiğini, %73,8’i bu uygulama derslerinin mesleki ilgilerini arttırdığını, %66,2’si ise öğrenimleri sürecinde motivasyonlarına olumlu katkı sağladığını bildirmişlerdir. Ayrıca, kadavranın yanısıra plastik model kullanımının öğrenme sürecini kolaylaştırıp bireysel çalışma verimini arttırdığı, ancak geleneksel bir seçenek olan kadavra tercihinin hala öğrenciler tarafından önemsendiği ve modellerin kadavra ile birlikte kullanımının tercih edildiği saptanmıştır. Veteriner elektrokardiyografi uygulaması ve kullanımının mesleki tecrübeye katkı sağlayacağı (%100), kazandırdığı tecrübenin önemli olduğu (%96,7), dersi ve uygulamayı daha iyi anlamayı sağladığı (%83,4) belirlenmiştir. TARTIŞMA ve SONUÇ: Laboratuvar olanaklarının ve alternatif eğitim metodlarının kullanılmasının öğrenci motivasyonu ve mesleki gelişim açısından Veteriner Hekimlik eğitimine olumlu katkısı olduğu kanısına varılmıştır.
