Nontyphoid Salmonella carriage, serovar profile and antimicrobial resistance phenotypes in slaughter cattle

Abstract

Current nontyphoid Salmonella (NTS) carriage in 200 apparently healthy slaughter cattle by ISO 6579 standard bacteriology (ISO) was 1% (2/200) in carcass and fecal content, and 2% (4/200) in mesenterial lymph nodes. There was no isolation from liver, kidney, spleen, and gallbladder, with an overall prevalence of 4% (8/200). Real-time PCR was in substantial agreement to ISO in confirming Salmonella-suspect isolates (Relative Trueness: 93.33%). Predominant serovar was S. Enteritidis (50%) followed by S. Typhimurium (37.5%), and S. Albany (12.5%). Five and three of eight NTS isolates were susceptible (62.5%) and resistant (37.5%) to 18 antimicrobials, respectively. Only three S. Enteritidis isolates (37.5%) showed multidrug resistance to 2-3 of 7 antimicrobials (amikacin, cefotaxime, cefoxitin, gentamicin, norfloxacin, pefloxacin, and tobramycin). S. Enteritidis predominance over S. Typhimurium, first detection of S. Albany in cattle in Turkey, and sole resistance in mesenterial lymph node S. Enteritidis isolates highlights study findings.