dc.contributor.author | Canbaz, Mehmet Çetin | |
dc.contributor.author | Sezgintürk, Mustafa Kemal | |
dc.date.accessioned | 2022-05-11T14:29:55Z | |
dc.date.available | 2022-05-11T14:29:55Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0003-2697 | |
dc.identifier.issn | 1096-0309 | |
dc.identifier.uri | https://doi.org/10.1016/j.ab.2013.10.014 | |
dc.identifier.uri | https://hdl.handle.net/20.500.11776/7172 | |
dc.description.abstract | Anti-HER-3 antibody was used for the first time in a disposable immunosensor based on indium tin oxide (ITO) substrate for HER-3 quantification. Anti-HER-3 was immobilized onto ITO substrate by 3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde. This highly sensitive immunosensor was capable of detecting concentrations of HER-3 down to the femtogram/ml level by investigating changes in the charge transfer resistance (R-ct) using electrochemical impedance spectroscopy (EIS). Construction of ITO layers was carefully investigated using a broad range of techniques such as voltammetry, EIS, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Meanwhile, in an immunosensor system, the single frequency impedance technique was first used for characterization of interaction between HER-3 and anti-HER-3. Eventually, the proposed ITO-based immunosensor was applied to artificial serum samples spiked with HER-3. (C) 2013 Elsevier Inc. All rights reserved. | en_US |
dc.description.sponsorship | TUBITAK (Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [109 T 172]; Namik Kemal University Scientific Research Projects FundNamik Kemal University [NKUBAP.00.10.YL.13.03]; Namik Kemal University Scientific and Technological Research CenterNamik Kemal University | en_US |
dc.description.sponsorship | This work was financially supported by TUBITAK (Scientific and Technological Research Council of Turkey, project 109 T 172). In addition, we thank the Namik Kemal University Scientific Research Projects Fund (project NKUBAP.00.10.YL.13.03) and the Namik Kemal University Scientific and Technological Research Center. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Academic Press Inc Elsevier Science | en_US |
dc.identifier.doi | 10.1016/j.ab.2013.10.014 | |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Cancer biomarkers | en_US |
dc.subject | Biosensor | en_US |
dc.subject | HER-3 | en_US |
dc.subject | ITO | en_US |
dc.subject | Single frequency impedance | en_US |
dc.subject | Breast-Cancer | en_US |
dc.subject | Expression | en_US |
dc.subject | Her3 | en_US |
dc.subject | Survival | en_US |
dc.subject | Prognosis | en_US |
dc.subject | C-Erbb-3 | en_US |
dc.subject | Protein | en_US |
dc.title | Fabrication of a highly sensitive disposable immunosensor based on indium tin oxide substrates for cancer biomarker detection | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Analytical Biochemistry | en_US |
dc.department | Fakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü | en_US |
dc.identifier.volume | 446 | en_US |
dc.identifier.startpage | 9 | en_US |
dc.identifier.endpage | 18 | en_US |
dc.institutionauthor | Canbaz, Mehmet Çetin | |
dc.institutionauthor | Sezgintürk, Mustafa Kemal | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.authorscopusid | 55928560800 | |
dc.authorscopusid | 6506764918 | |
dc.authorwosid | Sezgintürk, Mustafa Kemal/AAY-1439-2021 | |
dc.identifier.wos | WOS:000329949500002 | en_US |
dc.identifier.scopus | 2-s2.0-84887571584 | en_US |
dc.identifier.pmid | 24141078 | en_US |