dc.contributor.author | Sezgintürk, Mustafa Kemal | |
dc.contributor.author | Uygun, Zihni Onur | |
dc.date.accessioned | 2022-05-11T14:29:46Z | |
dc.date.available | 2022-05-11T14:29:46Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 0003-2697 | |
dc.identifier.issn | 1096-0309 | |
dc.identifier.uri | https://doi.org/10.1016/j.ab.2011.12.049 | |
dc.identifier.uri | https://hdl.handle.net/20.500.11776/7126 | |
dc.description.abstract | In the present work, we have developed a new biosensor based on fourth-generation (G4) PAMAM dendrimers for the analysis of vascular endothelial growth factor (VEGF). First, the PAMAM dendrimers were covalently attached to a cysteamine-modified Au electrode by glutaraldehyde. With the help of the amino groups located on its surface, vascular endothelial growth factor receptor-1 (VEGF-R1) was immobilized via glutaraldehyde cross-linking. VEGF-R1 loading was investigated to identify the optimal VEGF-R1 immobilization conditions for the best sensitivity of the new biosensor. In addition, Kramers-Kronig transforms were also analyzed for immobilization and measurement processes. The biosensor had a linear range of 5 to 125 pg/mL VEGF. The fabricated biosensor had good repeatability and reproducibility. Finally, the results for artificial serum samples measured by the present biosensor showed a good recovery for VEGF detection. (C) 2012 Elsevier Inc. All rights reserved. | en_US |
dc.description.sponsorship | TUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [109 T 172] | en_US |
dc.description.sponsorship | The authors gratefully acknowledge the support from TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 109 T 172). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Academic Press Inc Elsevier Science | en_US |
dc.identifier.doi | 10.1016/j.ab.2011.12.049 | |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | VEGF | en_US |
dc.subject | Tumor growth | en_US |
dc.subject | VEGF-R1 | en_US |
dc.subject | PAMAM | en_US |
dc.subject | Electrochemical impedance spectroscopy | en_US |
dc.subject | Biosensor | en_US |
dc.subject | Cancer Biomarker | en_US |
dc.subject | Breast-Cancer | en_US |
dc.subject | Electrical Detection | en_US |
dc.subject | Impedance Spectroscopy | en_US |
dc.subject | Factor Vegf | en_US |
dc.subject | Dna | en_US |
dc.subject | Mechanisms | en_US |
dc.subject | Expression | en_US |
dc.subject | Dispersion | en_US |
dc.subject | Sequence | en_US |
dc.title | An impedimetric vascular endothelial growth factor biosensor-based PAMAM/cysteamine-modified gold electrode for monitoring of tumor growth | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Analytical Biochemistry | en_US |
dc.department | Fakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü | en_US |
dc.authorid | 0000-0001-9045-7271 | |
dc.authorid | 0000-0001-9045-7271 | |
dc.identifier.volume | 423 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.startpage | 277 | en_US |
dc.identifier.endpage | 285 | en_US |
dc.institutionauthor | Sezgintürk, Mustafa Kemal | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.authorscopusid | 6506764918 | |
dc.authorscopusid | 51566114200 | |
dc.authorwosid | uygun, zihni onur onur/P-8783-2015 | |
dc.authorwosid | Sezgintürk, Mustafa Kemal/AAY-1439-2021 | |
dc.authorwosid | Uygun, Zihni Onur/M-2952-2019 | |
dc.identifier.wos | WOS:000302336100014 | en_US |
dc.identifier.scopus | 2-s2.0-84859009441 | en_US |
dc.identifier.pmid | 22266295 | en_US |