Vaccine Production Based on Oocyst Irradiation at Broiler Coccidiosis I: Studies on the Quantitative Determination and Sporulation of Oocysts in Faeces Present in Litter of Broilers with Coccidiosis

Abstract

The objective of this study was to evaluate a standard method for quantitative isolation of Eimeria oocysts from feces samples and effective sporulation. It's realized that contamination related with litter can effectively impress the result about quantitative determination of oocyst existance in faeces taken from hencoops that lots of chickens live together. This negativity can be arranged by performing pre-separation process for newly collected samples and with this process related problem could be solved to a certain degree. In the experiments made for determining the ideal perspective about oocyst sporulation, oxygen, motion and potassium dichromate (K(2)Cr(2)O(7)) were emphasized and the importance of potassium dichromate understood again. Moreover the obtained data showed that different reasons about potassium dichromate's action mechanism directly or indirectly could take part in sporulation.