H2O2'nin Bazı Ekmeklik Buğday (Triticum aestivum L.) Genotiplerinde Bazı Fide Dönemi Özelliklerine Etkisi

Abstract

The aim of this research was to determine the effect of oxidative stress derived from exogenous application of hydrogen peroxide (H2O2) onto some morpho-physiological characteristics of some bread wheat entries at seedling stages. In the research, 5 bread wheat cultivars (Flamura-85, Selimiye, Esperia, Tosunbey and Sultan-95) and 3 advanced bread wheat lines (TDE-45-1, TDE-84-5, TDE-111-9) were used as plant material. The experiment was carried out in randomized complete split plot design with 3 replicates in pots in controlled conditions where bread wheat genotypes were placed into main and H2O2 ratios (i.e. 0, 50 and 100 mu M) as sub plots respectively. Root and shoot length (cm), chlorophyll content (SPAD), the rate of leaf water loss (%), number of stomata (number), stomata width and length (mu) of the plants were recorded on 3rd day after H2O2 application. Root length, shoot length, chlorophyll content, stomata width and stomata length decreased 8.60-17.18 %, 5.85-11.36 %, 6.50-14.98 %, 12.00-13.77 % and 9.78-20.66 %, respectively, with oxidative stress created by H2O2 application. However, number of stomata and the rate of leaf water loss were increased 16.83-21.60% and 27.95-46.50%, respectively. It was concluded that the responses of Flamura-85, TDE-45-1 and TDE-111-9 were better responding entries to H2O2 based oxidative stress than the other genotypes for all traits used in the study.