dc.contributor.author | Karaboğa, Munteha Nur Sonuç | |
dc.contributor.author | Şimşek, Çiğdem Sayikli | |
dc.contributor.author | Sezgintürk, Mustafa Kemal | |
dc.date.accessioned | 2022-05-11T14:12:20Z | |
dc.date.available | 2022-05-11T14:12:20Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0956-5663 | |
dc.identifier.issn | 1873-4235 | |
dc.identifier.uri | https://doi.org/10.1016/j.bios.2015.08.044 | |
dc.identifier.uri | https://hdl.handle.net/20.500.11776/5511 | |
dc.description.abstract | This paper describes a novel, simple, and disposable immunosensor based on indium-tin oxide (ITO) sheets modified with gold nanoparticles to sensitively analyze heat shock protein 70 (HSP70), a potential biomarker that could be evaluated in diagnosis of some carcinomas. Disposable ITO coated Polyethylene terephthalate (PET) electrodes were used and modified with gold nanoparticles in order to construct the biosensors. Optimization and characterization steps were analyzed by electrochemical techniques such as electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Surface morphology of the biosensor was also identified by electrochemical methods, scanning electron microscopy (SEM), and atomic force microscopy (AFM). To interpret binding characterization of HSP70 to anti-HSP70 single frequency impedance method was successfully operated. Moreover, the proposed HSP70 immunosensor acquired good stability, repeatability, and reproducibility. Ultimately, proposed biosensor was introduced to real human serum samples to determine HSP70 sensitively and accurately. (C) 2015 Elsevier B.V. All rights reserved. | en_US |
dc.description.sponsorship | TUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113 Z 678] | en_US |
dc.description.sponsorship | The financial support from TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 113 Z 678) is gratefully acknowledged. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier Advanced Technology | en_US |
dc.identifier.doi | 10.1016/j.bios.2015.08.044 | |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Heat shock proteins | en_US |
dc.subject | HSP70 | en_US |
dc.subject | ITO | en_US |
dc.subject | Gold nanoparticles | en_US |
dc.subject | Immunosensor | en_US |
dc.subject | Heat-Shock Proteins | en_US |
dc.subject | Hsp70 | en_US |
dc.subject | Cancer | en_US |
dc.subject | Expression | en_US |
dc.subject | Immunosensor | en_US |
dc.subject | Chaperones | en_US |
dc.subject | Nanoparticles | en_US |
dc.subject | Sensors | en_US |
dc.title | AuNPs modified, disposable, ITO based biosensor: Early diagnosis of heat shock protein 70 | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Biosensors & Bioelectronics | en_US |
dc.department | Yüksekokullar, Sağlık Yüksekokulu, Beslenme ve Diyetetik Bölümü | en_US |
dc.department | Fakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü | en_US |
dc.authorid | 0000-0002-3153-1328 | |
dc.identifier.volume | 84 | en_US |
dc.identifier.startpage | 22 | en_US |
dc.identifier.endpage | 29 | en_US |
dc.institutionauthor | Karaboğa, Munteha Nur Sonuç | |
dc.institutionauthor | Şimşek, Çiğdem Sayikli | |
dc.institutionauthor | Sezgintürk, Mustafa Kemal | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.authorscopusid | 56800873300 | |
dc.authorscopusid | 56029590100 | |
dc.authorscopusid | 6506764918 | |
dc.authorwosid | Sezgintürk, Mustafa Kemal/AAY-1439-2021 | |
dc.authorwosid | Karaboga, Munteha Nur Sonuc/ABA-6419-2020 | |
dc.identifier.wos | WOS:000377313300004 | en_US |
dc.identifier.scopus | 2-s2.0-84940099597 | en_US |
dc.identifier.pmid | 26318579 | en_US |