dc.contributor.author | Sonuç Karaboğa, Münteha Nur | |
dc.contributor.author | Sezgintürk, Mustafa Kemal | |
dc.date.accessioned | 2022-05-11T14:12:19Z | |
dc.date.available | 2022-05-11T14:12:19Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 1573-4110 | |
dc.identifier.issn | 1875-6727 | |
dc.identifier.uri | https://doi.org/10.2174/1573411011666150603201229 | |
dc.identifier.uri | https://hdl.handle.net/20.500.11776/5497 | |
dc.description.abstract | This paper presents a new disposable electrochemical biosensor for the analysis of Haptoglobin (Hp) which shows many important functionalities such as antioxidant capabilities, anti-inflammatory activities and regulation of the immune system. Aminopropyltriethoxysilane (APTES) was self-assembled onto an indium tin oxide (ITO) substrate to fabricate a disposable biosensor. Anti-Hp antibodies were immobilized onto the modified ITO surface covalently. The optimization of the construction parameters and the analytical performance of the biosensor were examined and compared. The methods, cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (EIS) were operated for detailed characterization of the different surfaces of the biosensor. Kramers-Kronig Transform was also applied to raw EIS data for checking the verification of obtained experimental EIS data. Moreover, a useful method, single frequency impedance (SFI), was successfully operated to characterize the binding of Hp to the electrode surface modified with anti-Hp. At last, the presented biosensor was applied to the analysis of artificial serum samples, which were spiked with Hp. | en_US |
dc.description.sponsorship | TUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113 Z 678] | en_US |
dc.description.sponsorship | This work was funded by TUBITAK (The Scientific and Technological Research Council of Turkey, Project numbers: 113 Z 678) and this assistance is greatly acknowledged. Also we are grateful to NABILTEM (Namik Kemal University Scientific and Technological Research Center). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Bentham Science Publ Ltd | en_US |
dc.identifier.doi | 10.2174/1573411011666150603201229 | |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Haptoglobin | en_US |
dc.subject | indium tin oxide | en_US |
dc.subject | biosensor | en_US |
dc.subject | acute phase protein | en_US |
dc.subject | electrochemical impedance spectroscopy | en_US |
dc.subject | single frequency impedance | en_US |
dc.subject | Indium-Tin-Oxide | en_US |
dc.subject | Neuromyelitis-Optica | en_US |
dc.subject | Impedance Spectroscopy | en_US |
dc.subject | Porcine Haptoglobin | en_US |
dc.subject | Immunosorbent-Assay | en_US |
dc.subject | Cerebrospinal-Fluid | en_US |
dc.subject | Multiple-Sclerosis | en_US |
dc.subject | Serum Haptoglobin | en_US |
dc.subject | Messenger-Rna | en_US |
dc.subject | Expression | en_US |
dc.title | A Disposable, Highly Sensitive Biosensing System: Determination of Haptoglobin as a Significant Acute Phase Biomarker | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Current Analytical Chemistry | en_US |
dc.department | Rektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi | en_US |
dc.department | Fakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü | en_US |
dc.identifier.volume | 12 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.startpage | 43 | en_US |
dc.identifier.endpage | 53 | en_US |
dc.institutionauthor | Sonuç Karaboğa, Münteha Nur | |
dc.institutionauthor | Sezgintürk, Mustafa Kemal | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.authorscopusid | 55988499900 | |
dc.authorscopusid | 6506764918 | |
dc.authorwosid | Sezgintürk, Mustafa Kemal/AAY-1439-2021 | |
dc.identifier.wos | WOS:000362586800005 | en_US |
dc.identifier.scopus | 2-s2.0-85043641949 | en_US |