dc.contributor.author | Aydın, Muhammet | |
dc.contributor.author | Aydın, Elif Burcu | |
dc.contributor.author | Sezgintürk, Mustafa Kemal | |
dc.date.accessioned | 2022-05-11T14:07:23Z | |
dc.date.available | 2022-05-11T14:07:23Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 1616-5187 | |
dc.identifier.uri | https://doi.org/10.1002/mabi.201900109 | |
dc.identifier.uri | https://hdl.handle.net/20.500.11776/5088 | |
dc.description.abstract | In this study, an impedimetric immunosensor based on polymer poly(thiophene)-graft-poly(methacrylamide) polymer (P(Thi-g-MAm)) modified indium tin oxide (ITO) electrode is developed for the detection of the Neuron Specific Enolase (NSE) cancer biomarker. First, the P(Thi-g-MAm) polymer is synthesized and coated on the ITO electrode by using a spin-coating technique. P(Thi-g-MAm) polymer acts as an immobilization platform for immobilization of NSE-specific monoclonal antibodies. Anti-NSE antibodies are utilized as biosensing molecules and they bind to the amino groups of P(Thi-g-Mam) polymer via glutaraldehyde cross-linking. Spin-coating technique is employed for bioelectrode fabrication and this technique provides a thin and uniform film on the ITO electrode surface. This bioelectrode fabrication technique is simple and it generates a suitable platform for large-scale loadings of anti-NSE antibodies. This immunosensor exhibits a wide linear detection range from 0.02 to 4 pg mL?1 and with an ultralow detection limit of 6.1 fg mL?1. It reveals a good long-term stability (after 8 weeks, 78% of its initial activity), an excellent reproducibility (1.29% of relative standard deviation (RSD)), a good repeatability (5.55% of RSD), and a high selectivity. In addition, the developed immunosensor is proposed as a robust diagnostic tool for the clinical detection of NSE and other cancer biomarkers. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim | en_US |
dc.description.sponsorship | DS.18.180, NKUBAP.00 | en_US |
dc.description.sponsorship | This research was supported The Scientific and Technological Research Council of by Tekirdag? Nam?k Kemal University, NKU-BAP (Grant number: NKUBAP.00.DS.18.180). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Wiley-VCH Verlag | en_US |
dc.identifier.doi | 10.1002/mabi.201900109 | |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | disposable electrodes | en_US |
dc.subject | electrochemical impedance spectroscopy | en_US |
dc.subject | graft polymer | en_US |
dc.subject | lung cancer | en_US |
dc.subject | neuron-specific enolase | en_US |
dc.subject | Biomarkers | en_US |
dc.subject | Crosslinking | en_US |
dc.subject | Diseases | en_US |
dc.subject | Electrochemical impedance spectroscopy | en_US |
dc.subject | Graft copolymers | en_US |
dc.subject | Grafting (chemical) | en_US |
dc.subject | Immunosensors | en_US |
dc.subject | Monoclonal antibodies | en_US |
dc.subject | Neurons | en_US |
dc.subject | Plastic coatings | en_US |
dc.subject | Thiophene | en_US |
dc.subject | Tin oxides | en_US |
dc.subject | Disposable electrodes | en_US |
dc.subject | Glutaraldehyde cross-linking | en_US |
dc.subject | Impedimetric immunosensors | en_US |
dc.subject | Indium tin oxide electrodes | en_US |
dc.subject | Linear detection ranges | en_US |
dc.subject | Lung Cancer | en_US |
dc.subject | Neuron specific enolase | en_US |
dc.subject | Relative standard deviations | en_US |
dc.subject | Electrochemical electrodes | en_US |
dc.subject | copolymer | en_US |
dc.subject | indium tin oxide | en_US |
dc.subject | monoclonal antibody | en_US |
dc.subject | neuron specific enolase | en_US |
dc.subject | poly(thiophene) graft poly(methacrylamide) polymer | en_US |
dc.subject | tumor marker | en_US |
dc.subject | unclassified drug | en_US |
dc.subject | acrylamide derivative | en_US |
dc.subject | cross linking reagent | en_US |
dc.subject | enolase | en_US |
dc.subject | glutaraldehyde | en_US |
dc.subject | methacrylamide | en_US |
dc.subject | monoclonal antibody | en_US |
dc.subject | polymer | en_US |
dc.subject | polythiophene | en_US |
dc.subject | thiophene derivative | en_US |
dc.subject | tumor marker | en_US |
dc.subject | Article | en_US |
dc.subject | atomic force microscopy | en_US |
dc.subject | cyclic potentiometry | en_US |
dc.subject | energy dispersive X ray spectroscopy | en_US |
dc.subject | enzyme immobilization | en_US |
dc.subject | Fourier transform infrared spectroscopy | en_US |
dc.subject | human | en_US |
dc.subject | impedance spectroscopy | en_US |
dc.subject | limit of detection | en_US |
dc.subject | measurement | en_US |
dc.subject | protein cross linking | en_US |
dc.subject | proton nuclear magnetic resonance | en_US |
dc.subject | Raman spectrometry | en_US |
dc.subject | reproducibility | en_US |
dc.subject | scanning electron microscopy | en_US |
dc.subject | serum | en_US |
dc.subject | synthesis | en_US |
dc.subject | blood | en_US |
dc.subject | chemistry | en_US |
dc.subject | electrochemical analysis | en_US |
dc.subject | electrode | en_US |
dc.subject | genetic procedures | en_US |
dc.subject | immunoassay | en_US |
dc.subject | polymerization | en_US |
dc.subject | procedures | en_US |
dc.subject | Acrylamides | en_US |
dc.subject | Antibodies, Monoclonal | en_US |
dc.subject | Biomarkers, Tumor | en_US |
dc.subject | Biosensing Techniques | en_US |
dc.subject | Cross-Linking Reagents | en_US |
dc.subject | Electrochemical Techniques | en_US |
dc.subject | Electrodes | en_US |
dc.subject | Glutaral | en_US |
dc.subject | Humans | en_US |
dc.subject | Immunoassay | en_US |
dc.subject | Limit of Detection | en_US |
dc.subject | Phosphopyruvate Hydratase | en_US |
dc.subject | Polymerization | en_US |
dc.subject | Polymers | en_US |
dc.subject | Reproducibility of Results | en_US |
dc.subject | Thiophenes | en_US |
dc.title | A Highly Selective Poly(thiophene)-graft-Poly(methacrylamide) Polymer Modified ITO Electrode for Neuron Specific Enolase Detection in Human Serum | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Macromolecular Bioscience | en_US |
dc.department | Rektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi | en_US |
dc.identifier.volume | 19 | en_US |
dc.identifier.issue | 8 | en_US |
dc.institutionauthor | Aydın, Muhammet | |
dc.institutionauthor | Aydın, Elif Burcu | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.authorscopusid | 55571487900 | |
dc.authorscopusid | 57194273220 | |
dc.authorscopusid | 6506764918 | |
dc.identifier.wos | WOS:000479103500001 | en_US |
dc.identifier.scopus | 2-s2.0-85067867205 | en_US |
dc.identifier.pmid | 31222894 | en_US |