Improving of callus formation in 'Syrah'/'110R' combination by 5-chlorosalicylic acid and thidiazuron treatments

Abstract

Present study was performed to determine effects of two different chemicals on grafting parameters of Syrah/110R combination. For this aim, it was utilized from four doses (0, 1, 2 and 4 mM) of 5-chlorosalicylic acid (5-CSA) treatment and two doses (0 and 3 ppm) of thidiazuron (TDZ) treatment. After omega grafting was performed in the study, grafted cuttings were treated by different doses of 5-chlorosalicylic acid with or without thidiazuron. At the end of the study, it was observed that 5-chlorosalic acid treatments led to various results in attributes of shoot and omega grafting in grafted cuttings taken out from the callusing room and the best results were particularly recorded for thidiazuron added 5-chlorosalicylic acid treatments with the doses of 2 and 4 mM. As a result, 5-CSA 2 mM + TDZ 3 ppm and 5-CSA 4 mM + TDZ 3 ppm treatments caused satisfactory results in terms of callus formation at grafting point in Syrah/110R combination. © 2020 International Society for Horticultural Science. All rights reserved.