The Eff ects of the Basil (Ocimum sanctum) Treatment on the Tumor Necrosis Factor-α and Interleukin 1β Release in the Kidney Tissue of the Diabetic Rats

Abstract

This study aims to examine the changes of the Ocimum sanctum treatment on the tumor necrosis factor alpha (TNF-alpha) and interleukin 1 beta (IL-1 beta) in the kidney tissue of the rats, in which the experimental diabetes was induced with streptozotocin (STZ). Forty Sprague Dawley male rats were divided into 5 groups: Diabetes, Diabetes + Ocimum sanctum, Ocimum sanctum, Control, and Sham. The immunohistochemical localization of TNF-alpha and IL-1 beta in the kidney tissue was determined by using the streptavidin-biotin-peroxidase method. Strong TNF-alpha immunoreactivity was determined in the renal cortex of the rats in the Diabetes and Diabetes + Ocimum sanctum groups on 14th days, low immunoreactivity was determined in the rats in Ocimum sanctum, Sham, and Control groups. While strong IL-1 beta immunoreactivity was observed in the renal cortex of the Diabetes group, moderate IL-1 beta immunoreactivity was observed in the renal cortex of the Diabetes + Ocimum sanctum and low immunoreactivity was determined in the Ocimum sanctum, Sham, and Control groups. In this study, it was assessed how the polymorphisms, occurring in the cytokine genes of Ocimum sanctum in the rats, in which experimental diabetes was induced, and TNF-alpha and IL-1 beta, which was demonstrated to have an important role in the complication development in the diabetic patients affected the renal tissue.