dc.contributor.author | Şirin, Duygu Yaşar | |
dc.contributor.author | Yılmaz, İbrahim | |
dc.contributor.author | İşyar, Mehmet | |
dc.contributor.author | Öznam, Kadir | |
dc.contributor.author | Mahiroğulları, Mahir | |
dc.date.accessioned | 2022-05-11T14:28:36Z | |
dc.date.available | 2022-05-11T14:28:36Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 1305-8282 | |
dc.identifier.issn | 1309-0313 | |
dc.identifier.uri | https://doi.org/10.5606/ehc.2017.55186 | |
dc.identifier.uri | https://hdl.handle.net/20.500.11776/6887 | |
dc.description.abstract | Objectives: This study aims to investigate the possible effects of leukocyte concentration in the content of platelet-rich plasma (PRP) and the administration of PRP using a drug delivery system on chondrocyte proliferation in vitro conditions. Patients and methods: Blood from nine male patients (mean age 65 years; range 49 to 81 years) with advanced stage osteoarthritis who had not responded to medical or conservative treatments and underwent total knee arthroplasty was used to prepare two formulations: PRP with low concentration leukocytes (2000-4000 leukocytes/mu L) was designated as pure PRP (P-PRP), whereas PRP with high concentration leukocytes (9000-11000 leukocytes/mu L) as leukocyte-rich PRP (L-PRP). Samples were divided into five groups as control group (group 1), chondrocyte cultures with P-PRP applied directly (group 2), chondrocyte cultures with L-PRP applied directly (group 3), chondrocytes co-cultured with P-PRP applied hydrogel (group 4), and chondrocytes co-cultured with L-PRP applied hydrogel (group 5). In all groups; cell morphology, viability and proliferation were compared with the expression of stage-specific embryonic antigen-1 (SSEA-1), a precondrocyte marker. Results: Maximum cell proliferation and SSEA-1 expression occurred in group 4, with a statistically significant correlation between SSEA-1 expression and cell proliferation. Conclusion: Our study showed the importance of leukocyte concentration of PRP and efficiency of delivery systems such as hydrogel and that L-PRP administered with a delivery system is more efficient than conventional applications of PRP in the treatment of cartilage damage. | en_US |
dc.description.sponsorship | Namik Kemal University Scientific Research Administration Division [NKUBAP.00.10.AR.15.08] | en_US |
dc.description.sponsorship | This study was supported by grants (Project no: NKUBAP.00.10.AR.15.08) from Namik Kemal University Scientific Research Administration Division. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Turkish Joint Diseases Foundation | en_US |
dc.identifier.doi | 10.5606/ehc.2017.55186 | |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Co-culture techniques | en_US |
dc.subject | hydrogel | en_US |
dc.subject | platelet-rich plasma | en_US |
dc.subject | stage-specific embryonic antigens | en_US |
dc.subject | Cartilage | en_US |
dc.subject | Formulations | en_US |
dc.subject | Therapy | en_US |
dc.subject | Repair | en_US |
dc.subject | Cells | en_US |
dc.subject | Bone | en_US |
dc.title | Does leukocyte-poor or leukocyte-rich platelet-rich plasma applied with biopolymers have superiority to conventional platelet-rich plasma applications on chondrocyte proliferation? | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Eklem Hastaliklari Ve Cerrahisi-Joint Diseases and Related Surgery | en_US |
dc.department | Fakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü | en_US |
dc.authorid | 0000-0003-2003-6337 | |
dc.identifier.volume | 28 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.startpage | 142 | en_US |
dc.identifier.endpage | 151 | en_US |
dc.institutionauthor | Şirin, Duygu Yaşar | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.authorscopusid | 56769801000 | |
dc.authorscopusid | 35197435300 | |
dc.authorscopusid | 56258081800 | |
dc.authorscopusid | 56891784100 | |
dc.authorscopusid | 56002899200 | |
dc.authorwosid | sirin, duygu yasar/AAR-8685-2020 | |
dc.authorwosid | YILMAZ, Ibrahim/H-6199-2019 | |
dc.authorwosid | Mahirogullari, Mahir/AAA-4742-2020 | |
dc.identifier.wos | WOS:000415126000002 | en_US |
dc.identifier.scopus | 2-s2.0-85034021288 | en_US |
dc.identifier.pmid | 29125811 | en_US |