Effects of inoculant preparation time and doses on fermentation and aerobic stability characteristics of the second crop maize silages

Abstract

This study was carried out to determine the effects of inoculant preparation time and doses on fermenta tion and aerobic stability characteristics of the second crop maize ensiled under laboratory conditions. Maize was harvested at the dough maturity stage. MICROBIOS (Cuprem®, USA) was used as lactic acid bacteria+enzyme mixture silage inoculant. The inoculant was applied at 1.0×105 cfu/g (RD1), a recommended dose, and 2.0×105 cfu/g (DD1) double the recommended dose. The chopped forages were ensiled in 1.0-l anaerobic jars (Weck, Wher-Oftlingen, Germany) equipped with a lid that enables only gas release. Three jars per treatment were sampled on days 4, 7, 14, 21 and 55. The jars were stored at 20-22°C under laboratory conditions. After 55 days of ensiling, the silages were subjected to an aerobic stability test for 5 days. The study showed that doubling the rate of inoculant application was not effective than the recommended rate at enhancing the silage quality or aerobic stability. Moreover, preparation time did not improve the fermentation and aerobic stability of the second crop maize silage.

inokulant haziriama süresi ve dozun un h amur o lum döneminde hasat edilen misirsilajm m fermantasyon gelijimi üzerine etkilerinin laboratuar ko ju lan nda saptanmasi amaci ile düzenlenen çaliçmada, laktik asit+enzim silaj inokulanti olarak MiCROBIOS (Cuprem', USA)kullanilmi5tir. inokulant silajlara l.OxlO^cfu/g ve 2.0 x 10^ cfu/g düzeyinde kat,lm,5tir. Uygulamalardan sonra muameleleryalnizca gaz çikiçina olanak tan,yan, 1,0 I i trel i k özel kavanozlarasilolanmiftir. Kavanozlarlaboratuvarkojullannda 20-22°C'dedepolanmi5lardir. Silolamadan sonraki 4., 7., 14., 21. ve 55. günlerde hergruptan 3'erkavanozaçilmi5tir. Silolama dönemininsonunda açilan tüm silajlara 5 gün sure ile aerobik stabilité testi (20-22 C) uygulanm,5t,r. Sonuç olarak, ikinci ürün misirsilajarinda dozun a rtinlmasi ve bekletmesüresinin silaj fermantasyonu ve aerobik stabilité üzerinde olumlu bir etkisi gözlenmemiştir.