Isolation, Identification of Heat Resistant Moulds in Margarine and Determination of Their Heat Resistance

Abstract

In this study, moulds that cause problems in a margarine production plant which is located in Trakya region have been isolated and identified. In addition to, their heat resistance and lipolytic activity were studied. For this purpose, margarine samples from various production lots and process water samples from production plant were taken aseptically, transported immediately to the laboratory and analyzed. In this research, two different heat resistant mould strains have been isolated from margarines and process water. After identification of this moulds, their heat resistances at different temperatures have been investigated. Mould isolates were identified as heat resistant Aspergillus fumigatus, Paecilomyces variotii based on macroscopic and microscopic features. To this analyses results about thermal resistance, Aspergillus fumigatus and Paecilomyces variotii were ability to survive heat treatment at 95 degrees C 10 minutes and 90 degrees C 10 minutes, respectively.