dc.contributor.author | Alcay, Selim | |
dc.contributor.author | Toker, Mehmed Berk | |
dc.contributor.author | Gökçe, Elif | |
dc.contributor.author | Önder, Nail Tekin | |
dc.contributor.author | Üstüner, Burcu | |
dc.contributor.author | Nur, Zekariya | |
dc.date.accessioned | 2022-05-11T14:42:15Z | |
dc.date.available | 2022-05-11T14:42:15Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 1300-6045 | |
dc.identifier.uri | https://doi.org/10.9775/kvfd.2018.20843 | |
dc.identifier.uri | https://hdl.handle.net/20.500.11776/9303 | |
dc.description.abstract | The objective of the study was to determine the optimum concentration of BSA in lecithin-based extender for post-thawing quality and incubation resilience (0 h, 6 h and 10 h) of ram sperm. Ejaculates were collected from five rams via electro ejaculation. Ejaculates were mixed to obtain pooled semen.Then, pooled semen was diluted with soybean lecithin-based extender without BSA (control) or supplemented with different concentrations of BSA (2.5 mg/mL, 5 mg/mL, 7.5 mg/mL and 10 mg/mL), at a final concentration of 150x106 spermatozoon/mL. Sperm motility, plasma membrane functional integrity (HOST), mitochondrial activity (rhodamine123), capacitation status (CTC), and DNA integrity (TUNEL) were evaluated. At the 10 h incubation, motility, plasma membrane functional integrity and mitochondria! function were better preserved in the BSA5 group compared to the control group. It was determined that high doses of BSA (5 mg/mL, 7.5 mg/mL and 10 mg/mL) affected acrosome reaction.The highest acrosome reaction rates were obtained in BSA10 groups in 6 h and 10 h incubation (P<0.05). TUNEL assay demonstrated that there were no differences among groups for DNA fragmentation at post-thaw and during incubation periods. The study shows that BSA supplemented extenders may have beneficial effect on ram semen parameters at 0 h, 6 h and 10 h of incubation. The results of the present study demonstrated a remarkable advantage of using 5 mg/mL of BSA in 1% lecithin-based extender. | en_US |
dc.description.sponsorship | Uludag University Scientific Research Projects Unit, Bursa, Turkey, (BAP)Uludag University [KUAP (V)-2015/55] | en_US |
dc.description.sponsorship | This work was supported by the Uludag University Scientific Research Projects Unit, Bursa, Turkey, (BAP) (Project number: KUAP (V)-2015/55 | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Kafkas Univ, Veteriner Fakultesi Dergisi | en_US |
dc.identifier.doi | 10.9775/kvfd.2018.20843 | |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Bovine serum albumin | en_US |
dc.subject | Cryopreservation | en_US |
dc.subject | Incubation resilience | en_US |
dc.subject | Ram spermatozoa | en_US |
dc.subject | Soybean Lecithin | en_US |
dc.subject | Egg-Yolk | en_US |
dc.subject | Sperm Capacitation | en_US |
dc.subject | Dna Fragmentation | en_US |
dc.subject | Liquid Storage | en_US |
dc.subject | Quality | en_US |
dc.subject | Antioxidants | en_US |
dc.subject | Spermatozoa | en_US |
dc.subject | Motility | en_US |
dc.subject | Cryopreservation | en_US |
dc.title | Long Term Incubation Resilience of Post-Thaw Ram Semen Diluted with Lecithin-Based Extender Supplemented with Bovine Serum Albumin | en_US |
dc.title.alternative | Sığır Serum Albumini İlave Edilmiş Lesitin Bazlı Sulandırıcı İle Sulandırılan Koç Spermasının Dondurma-Çözdürme Sonrası Uzun Süreli İnkübasyon Direnci] | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Kafkas Universitesi Veteriner Fakultesi Dergisi | en_US |
dc.department | Fakülteler, Veteriner Fakültesi, Klinik Bilimler Bölümü, Dölerme ve Suni Tohumlama Ana Bilim Dalı | en_US |
dc.authorid | 0000-0002-7678-3289 | |
dc.identifier.volume | 25 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.startpage | 291 | en_US |
dc.identifier.endpage | 297 | en_US |
dc.institutionauthor | Gökçe, Elif | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.authorscopusid | 56099810300 | |
dc.authorscopusid | 56480349200 | |
dc.authorscopusid | 56779799700 | |
dc.authorscopusid | 55670728900 | |
dc.authorscopusid | 18937724600 | |
dc.authorscopusid | 6508060684 | |
dc.authorwosid | Gokce, Elif/ABA-6294-2020 | |
dc.authorwosid | Ustuner, Burcu/AAG-7238-2021 | |
dc.authorwosid | Nur, Zekariya/AAH-2635-2021 | |
dc.identifier.wos | WOS:000460503100002 | en_US |
dc.identifier.scopus | 2-s2.0-85067432770 | en_US |