Voltammetric behavior of lercanidipine and anodic adsorptive stripping voltammetric method for assay in pharmaceutical dosage forms and biological fluids

Abstract

Electrochemistry of lercanidipine (LCN) was investigated and its optimal conditions were evaluated based on the irreversible and diffusion-controlled electrochemical oxidation signal (+ 0.93 V) of a carbon paste electrode (CPE) versus Ag/AgCl in Britton Robinson buffer (BR) and ethanol mixture of pH 4.5. Electrochemical determination was performed by square wave anodic adsorptive stripping voltammetry (SWAAdSV). Linear range was found to be between 3.3 x 10(-7) mol L-1 and 4.5 x 10(-5) mol L-1 in two different regions; preconcentration potential and time were found to be 0.0 V and 150 s, respectively. In this method, the limit of quantification (LOQ) was found to be 2.0 x 10(-8) mol L-1 (0.012 mg L-1). The method was applied to determine the content of LCN in a commercial pharmaceutical preparation, spiked human serum and spiked human urine. The method was found to be highly accurate and precise, having a relative standard deviation of less than 10% for all applications.