dc.contributor.author | Canbaz, Mehmet Çetin | |
dc.contributor.author | Şimşek, Çiğdem Sayikli | |
dc.contributor.author | Sezgintürk, Mustafa Kemal | |
dc.date.accessioned | 2022-05-11T14:29:54Z | |
dc.date.available | 2022-05-11T14:29:54Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0003-2670 | |
dc.identifier.issn | 1873-4324 | |
dc.identifier.uri | https://doi.org/10.1016/j.aca.2014.01.041 | |
dc.identifier.uri | https://hdl.handle.net/20.500.11776/7169 | |
dc.description.abstract | We have developed a new immunological biosensor for ultrasensitive quantification of human epidermal growth factor receptor-3(HER-3). In order to construct the biosensor, the gold electrode surface was layered with, hexanedithiol, gold nanoparticles, and cysteamine, respectively. Anti-HER-3 antibody was covalently attached to cysteamine by glutaraldehyde and used as a bioreceptor in a biosensor system for the first time by this study. Surface characterization was obtained by means of electrochemical impedance spectroscopy and voltammetry. The proposed biosensor showed a good analytical performance for the detection of HER-3 ranging from 0.2 to 1.4 pg mL(-1). Kramers-Kronig transform was performed on the experimental impedance data. Moreover, in an immunosensor system, the single frequency impedance technique was firstly used for characterization of interaction between HER-3 and anti-HER-3. Finally the presented biosensor was applied to artificial serum samples spiked with HER-3. (C) 2014 Elsevier B.V. All rights reserved. | en_US |
dc.description.sponsorship | TUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [109T 172] | en_US |
dc.description.sponsorship | This work was supported by a grant from TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 109T 172). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier Science Bv | en_US |
dc.identifier.doi | 10.1016/j.aca.2014.01.041 | |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Cancer biomarkers | en_US |
dc.subject | HER-3 | en_US |
dc.subject | Anti-HER-3 | en_US |
dc.subject | Single frequency impedance | en_US |
dc.subject | Biosensor | en_US |
dc.subject | Growth-Factor Receptor | en_US |
dc.subject | Breast-Cancer | en_US |
dc.subject | Expression | en_US |
dc.subject | Dispersion | en_US |
dc.subject | Antibody | en_US |
dc.subject | Therapy | en_US |
dc.subject | Family | en_US |
dc.title | Electrochemical biosensor based on self-assembled monolayers modified with gold nanoparticles for detection of HER-3 | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Analytica Chimica Acta | en_US |
dc.department | Fakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü | en_US |
dc.identifier.volume | 814 | en_US |
dc.identifier.startpage | 31 | en_US |
dc.identifier.endpage | 38 | en_US |
dc.institutionauthor | Canbaz, Mehmet Çetin | |
dc.institutionauthor | Şimşek, Çiğdem Sayikli | |
dc.institutionauthor | Sezgintürk, Mustafa Kemal | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.authorscopusid | 55928560800 | |
dc.authorscopusid | 56029590100 | |
dc.authorscopusid | 6506764918 | |
dc.authorwosid | Sezgintürk, Mustafa Kemal/AAY-1439-2021 | |
dc.identifier.wos | WOS:000331556400003 | en_US |
dc.identifier.scopus | 2-s2.0-84893642278 | en_US |
dc.identifier.pmid | 24528841 | en_US |